王亮,周子娟,詹红微,雒东,陈大朋,王靖宇
WANG Liang,ZHOU Zi-juan,LI Ya-chan,LUO Dong,CHEN Da-peng,WANG Jing-yu

• 1:大连医科大学实验动物中心

摘要(Abstract)：

目的研究麦冬皂苷D(ophiopogonin D,OPD)对小肠上皮细胞损伤的保护作用及其作用机制。方法体外培养大鼠小肠上皮细胞IEC-6,用噻唑兰(methylthiazolyldiphenyl-tetrazolium bromide,MTT)法检测麦冬皂苷D对大鼠小肠上皮细胞IEC-6活性的影响;建立细菌脂多糖(lipopolysaccharides,LPS)损伤大鼠小肠上皮细胞IEC-6模型,检测麦冬皂苷D对上皮细胞的凋亡作用以及紧密连接屏障功能作用的影响。结果 LPS可显著降低IEC-6细胞的存活率;LPS显著促进炎症指标核转录因子NF-κB、凋亡相关蛋白caspase-9以及可导致紧密连接功能紊乱的肌球蛋白轻链激酶(myosin light chain kinase,MLCK)的表达;LPS抑制抗凋亡蛋白Bcl-2的表达。麦冬皂苷D可逆转LPS造成的功能紊乱,包括促进Bcl-2的表达,抑制caspase-3及MLCK的表达,一定程度上恢复黏膜屏障功能。结论麦冬皂苷D可缓解LPS造成的肠上皮细胞损伤,该作用与抗炎、抑制细胞凋亡及降低MLCK表达相关;麦冬皂苷D可能成为炎症性肠病临床治疗的一个潜在的候选药物。
Objective To investigate the protective effect and mechanisms of ophiopogonin D on intestinal epithelial cell injury.Methods The rat intestinal epithelial IEC-6 cells were cultured and the MTT assay was used to determine the effects of the ophiopogonin D on IEC-6 cell activity.The IEC-6 cell injury model was established through incubation of cells with bacterial lipopolysaccharides(LPS).The effects of ophiopogonin D on apoptosis and tight junction barrier function were respectively studied.Results LPS significantly reduced IEC-6 cell viability;LPS significantly induced the high expression of NF-κB which is one of inflammatory markers,apoptosis-related protein caspase-9and myosin light chain kinase(MLCK)which can lead to tight junction barrier dysfunciton;LPS also inhibited the expression of anti-apoptotic protein Bcl-2 expression.ophiopogonin D significangly reversed the effects induced by LPS,including upregulation of Bcl-2,inhibition of caspase-3 and MLCK,finally lead to restoration of barrier function.Conclusions ophiopogonin D alleviates the epithelial cell injury induced by LPS through the anti-inflammatory,inhibition of cell apoptosis and downregulation of MLCK.ophiopogonin D might be a potential candidate drug for the clinical treatment of inflammatory bowel disease.

关键词(KeyWords)： 麦冬皂苷D;炎性肠病;肠黏膜屏障;肌球蛋白轻链激酶
ophiopogonin D;inflammatory bowel disease;intestinal mucosal barrier;myosin light chain kinase

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(81600440)

作者(Author): 王亮,周子娟,詹红微,雒东,陈大朋,王靖宇
WANG Liang,ZHOU Zi-juan,LI Ya-chan,LUO Dong,CHEN Da-peng,WANG Jing-yu

DOI: 10.14066/j.cnki.cn21-1349/r.2017.06.011

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