L-缬氨酸高产菌选育及营养需求研究Study on the breeding of L-valine hyper-producer and its requirement for nutrients
张伟国,钱和
ZHANG Wei guo; QIAN He (Southern Yangtze University;
摘要(Abstract)：
以黄色短杆菌 (Brevibacteriumflavum)L 缬氨酸产生菌ZQ -2为出发菌株 ,经硫酸二乙酯(DES)和亚硝基胍 (NTG)诱变处理 ,氨基酸结构类似物定向筛选 ,获得一株L 缬氨酸高产菌XQ -6 (LeulAHVrα ABhr2 TAhr)。在以 1 4 %葡萄糖为碳源、5 %硫酸铵为氮源的培养基中直接发酵72h,产酸达 5 8～ 6 2g/L。同时添加Fe2 + 和Mn2 + 比单独添加Fe2 + 更有效。还进行了氨基酸、核酸、维生素和其它营养对L 缬氨酸发酵影响的试验。
Using Brevibacterium flavum ZQ 2 (Leul 1α AB rAHV r2 TA r) as a starting strain for mutagenesis by diethylsulfate (DES), N methyl N ′ nitro soguanidine (NTG) treatment, a L valine hyper producer XQ 6 (Leu 1 AHV r α AB hr 2 TA hr ) was obtained, which could be resistant to high concentration of α amino butyric acid (α AB) and 2 thiazolealanaine (2 TA). The strain was cultured with shaking at (31±1)℃ for 72 h , it could accumulate 58～ 62 g/L of L valine in a medium containing 14% glucose and 5% (NH 4) 2SO 4.The addition of Fe 2+ and Mn 2+ at the same time gave much more productivity than that of Fe 2+ alone. Effects of amino acids, nucleic acids, vitamins, and other nutrients on L valine fermentation were investigated.
关键词(KeyWords)：
L-缬氨酸;诱变育种;发酵;黄色短杆菌
L valine; breeding; fermentation; Brevibacterium flavum
基金项目(Foundation):
作者(Authors):
张伟国,钱和
ZHANG Wei guo; QIAN He (Southern Yangtze University;
参考文献(References)：
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