吴秀丽,张怡轩,赵文倩,王金辉,吴春福,李铣
WU Xiu-li1,ZHANG Yi-xuan2,ZHAO Wen-qian2,WANG Jin-hui1,WU Chun-fu2,LI Xian1(1.School of Traditional Chinese Materia Medica

• 1:沈阳药科大学中药学院
• 2:沈阳药科大学生命科学与生物制药学院
• 3:沈阳药科大学生命科学与生物制药学院
• 4:沈阳药科大学中药学院

摘要(Abstract)：

目的筛选能将人参皂苷Rg1定向转化为稀有人参皂苷F1的真菌。方法通过硅胶柱色谱从三七总皂苷中分离纯化出人参单体皂苷Rg1;采用固体与液体发酵培养法,从325株真菌中筛选到能对单体人参皂苷Rg1进行生物转化的菌株。采用1H-NMR1、3C-NMR波谱手段及理化性质鉴定人参皂苷Rg1及其转化产物。结果发现真菌菌株EST-Ⅰ与EST-Ⅱ将人参皂苷Rg1定向转化为人参皂苷F1,说明两种菌株能产生特异性水解C-6位葡萄糖的β-葡萄糖苷酶。结论该方法是一种制备稀有人参皂苷F1的新方法。
Objective To screen the fungi strains which can specifically transform ginsenoside Rg1 to the rare ginsenoside F1.Methods The ginsenoside Rg1 was separated and purified from the total saponins of Panax notoginseng by column chromatography on silica gel;Through solid and liquor transformation cultures screening from 325 fungi strains,two strains EST-Ⅰ and EST-Ⅱ were found which can convert ginsenoside Rg1 efficiently into ginsenoside F1.The structures of ginsenoside Rg1 and its microbiological transformation product were identified by spectral analysis(1H-NMR,13C-NMR)and physico-chemical properties.Results The fungi strains EST-Ⅰ and EST-Ⅱ could convert ginsenoside Rg1 into ginsenoside F1,which showed that they could produce specific C6-β-glucosidase.Conclusions The studies find out a new preparation method of the rare ginsenoside F1.

关键词(KeyWords)： 生物转化;人参皂苷F1;人参皂苷Rg1;真菌
biotransformation;ginsenosides F1;ginsenosides Rg1;fungus

Abstract:

Keywords:

基金项目(Foundation): 国家科技基础条件平台建设项目(2005DKA21203);; 教育部跨世纪优秀人才支持计划(NCET-04-0289);; 国家863支持计划(2002AA2Z3231)

作者(Author): 吴秀丽,张怡轩,赵文倩,王金辉,吴春福,李铣
WU Xiu-li1,ZHANG Yi-xuan2,ZHAO Wen-qian2,WANG Jin-hui1,WU Chun-fu2,LI Xian1(1.School of Traditional Chinese Materia Medica

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