HPLC法同时测定舒胆胶囊中芦荟大黄素和延胡索乙素的含量HPLC simultaneous determination of aloe-emodin and tetrahydropalmatine in Shudan capsules
陈丽姑,王进,王冬梅,赵怀清
CHEN Li-gu1,WANG Jin2,WANG Dong-mei1,ZHAO Huai-qing1 (1.School of Pharmacy
摘要(Abstract)：
目的建立同时测定舒胆胶囊中芦荟大黄素和延胡索乙素含量的方法,为完善现有质量标准提供科学依据。方法采用HPLC法。用Diamonsil C18柱(200 mm×4.6 mm,5μm)分离,以甲醇-pH6.8磷酸盐缓冲液(体积比70∶30)进行等度洗脱,流速为1.0 mL.min-1,柱温为40℃,检测波长为280 nm。结果芦荟大黄素质量浓度在0.130 0~2.600 mg.L-1内、延胡索乙素质量浓度在4.352~87.04 mg.L-1内与峰面积呈良好的线性关系,相关系数分别为0.999 7和0.999 9;平均回收率分别为100.0%(RSD=0.81%)和100.1%(RSD=0.33%)。结论本方法简便、快捷,结果准确,重复性好,可应用于舒胆胶囊的质量控制。
Objective To establish an HPLC method for simultaneous determination of aloe-emodin and tetrahydropalmatine in Shudan(traditional Chinese medicines)capsules.Methods The chromatographic method was applied on a Diamonsil C18 column(200 mm×4.6 mm,5 μm) with methanol-pH 6.8 phosphate buffer(V∶V=70∶30) as the mobile phase.The flow rate was 1.0 mL·min-1 and column temperature was 40 ℃.The detection wavelength was set at 280 nm.Results The calibration curve was linear within the range of 0.130 0-2.600 mg·L-1(r=0.999 7) for aloe-emodin and 4.352-87.04 mg·L-1(r=0.999 9) for tetrahydropalmatine;the average recoveries were 100.0 %(RSD=0.81%) for aloe-emodin and 100.1 %(RSD=0.33%) for tetrahydropalmatine.Conclusions The method is simple,accurate and reproducible and can be used for the quality control of Shudan capsules.
关键词(KeyWords)：
舒胆胶囊;芦荟大黄素;延胡索乙素;高效液相色谱法
Shudan capsule;aloe-emodin;tetrahydropalmatine;HPLC
基金项目(Foundation):
作者(Author):
陈丽姑,王进,王冬梅,赵怀清
CHEN Li-gu1,WANG Jin2,WANG Dong-mei1,ZHAO Huai-qing1 (1.School of Pharmacy
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