张起辉;周莲娣;闫小玉;陈刚;田黎;裴月湖;
ZHANG Qi-hui1,ZHOU Lian-di2,YAN Xiao-yu1,CHEN Gang1,TIAN Li3,PEI Yue-hu1(1.School of Traditional Chinese Materia Medica,Shenyang Pharmaceutical University,Shenyang 110016,China;2.Faculty of Medicine,Chongqing Medical University,Chongqing 400016,China;3.Department of Biological Pharmacy,Qingdao University of Science and Technology,Qingdao 266042,China)

• 1:沈阳药科大学中药学院
• 2:重庆医科大学基础医学院
• 3:青岛科技大学生物制药系

摘要(Abstract)：

目的对海洋真菌Nigrospora sphaerica中的化学成分进行研究。方法采用硅胶柱色谱、凝胶以及HPLC等方法进行分离纯化,根据理化性质和光谱数据进行结构鉴定。结果分离得到10个化合物,分别鉴定为尿嘧啶(uracil,1)、胸腺嘧啶(thymine,2)、半乳糖醇(D-galactitol,3)、5α,8α-过氧麦角甾-6,22E-二烯-3β-醇(5α,8α-epidioxy-(22E,24R)-ergosta-6,22E-dien-3β-ol,4)、5α,8α-过氧麦角甾-6,9,22E-三烯-3β-醇(5α,8α-epidioxy-(22E,24R)-ergosta-6,9,22E-triere-3β-ol,5)、丁二酸(succinic acid,6)、4-(2-羟乙基)苯酚(4-(2-hydroxyethyl)phenol,7)、3-异丁基-7-羟基-吡咯并哌嗪-1,4-二酮(3-isobutyl-7-hydroxy-pyrrolopiperazine-1,4-dione,8)、对羟基苯甲酸(4-hydroxybenzoicacid,9)和苯乙酸(phenylacetic acid,10)。结论海洋真菌Nigrospora sphaerica为首次从海洋中分离得到,化合物4¹0为首次从该真菌中分离得到。
Objective To study chemical constituents from the marine Nigrospora sphaerica.Methods The compounds were isolated by chromatography on silica gel columns,Sephadex LH-20 and HPLC,and then identified by their physical-chemical properties and spectral analysis.Results Ten compounds were obtained and identified as uracil(1),thymine(2),D-galactitol(3),5α,8α-epidioxy-(22E,24R)-ergosta-6,22E-dien-3β-ol(4),5α,8α-epidioxy-(22E,24R)-ergosta-6,9,22E-triere-3β-ol(5),succinic acid(6),4-(2-hydroxyethyl)phenol(7),3-isobutyl-8-hydroxy-pyrrolo-piperazine-1,4-dione(8),4-hydroxybenzoic acid(9),phenylacetic acid(10),respectively.Conclusions The Nigrospora sphaerica is obtained from marine for the first time,compounds 410 are isolated from the marine fungi for the first time.

关键词(KeyWords)： 海洋真菌Nigrospor asphaerica;化学成分;结构鉴定
marine Nigrospora sphaerica;chemical constituent;structure identification

Abstract:

Keywords:

基金项目(Foundation): 国家863支持项目(2007AA09Z413)

作者(Authors): 张起辉;周莲娣;闫小玉;陈刚;田黎;裴月湖;
ZHANG Qi-hui1,ZHOU Lian-di2,YAN Xiao-yu1,CHEN Gang1,TIAN Li3,PEI Yue-hu1(1.School of Traditional Chinese Materia Medica,Shenyang Pharmaceutical University,Shenyang 110016,China;2.Faculty of Medicine,Chongqing Medical University,Chongqing 400016,China;3.Department of Biological Pharmacy,Qingdao University of Science and Technology,Qingdao 266042,China)

DOI: 10.14066/j.cnki.cn21-1349/r.2010.05.009

参考文献(References)：

• [1]龚运淮,丁立生.天然产物核磁共振碳谱分析[M].2版.云南:云南科技出版社,2005:884.
• [2]汤海峰,易杨华,姚新生,等.褐藻铁钉菜中的甾醇成分[J].中国海洋药物,2002(1):1-4.
• [3]PINCHUK I V,BRESSOLLIER P S,SOROKULOVAI B,et al.Amicoumacin antibiotic production and ge-netic diversity ofBacillussubtilis strains isolated fromdifferent habitats[J].Research in Micro,2002,153(5):269-276.
• [4]于德泉,杨峻山.分析化学手册:第7分册[M].北京:化学工业出版社,1996:65-66.
• [5]JAYATILAKE G S,THOMTON M P,LEONERD AC,et al.Metabolites from anAntarcticsponge-associat-ed bacterium pseudomonas[J].J Nat Prod,1996,59(3):293-296.
• [6]黄洪波,包文芳,杨芳芳,等.灯盏花的化学成分研究[J].沈阳药科大学学报,2001,18(4):266-267.