王芳,张亚宏,左代英,吴英良
WANG Fang1,ZHANG Ya-hong2,ZUO Dai-ying2,WU Ying-liang2(1.Library

• 1:沈阳药科大学图书馆
• 2:沈阳药科大学生命科学与生物制药学院

摘要(Abstract)：

目的进行红人归胶囊(HRG)治疗更年期综合征机制的研究。方法采用去势雌性(ovariec-tomized,OVX)大鼠作为更年期动物模型,放射性免疫法测定OVX大鼠血清雌二醇含量,高效液相色谱-电化学检测法测定下丘脑单胺神经递质及其代谢产物的含量,并测定其免疫器官的脏器系数。结果HRG可使OVX大鼠血清雌二醇含量升高,使下丘脑升高的5-羟色胺(5-HT)及其代谢产物5-羟色胺酸(5-HIAA)含量明显下降,显著提高去甲肾上腺素(NE)和多巴胺(DA)含量,使升高的5-HT含量与NE含量的比值降低至接近正常;HRG能提高OVX大鼠胸腺系数和脾系数。结论HRG通过调节植物神经功能、生殖内分泌系统和免疫功能,多层次、多途径、多环节地作用于更年期衰退的神经内分泌免疫网络,从而起到稳定机体内环境,缓解更年期症状的作用。
Objective To study the action mechanisms of Hongrengui capsules(HRG,traditional Chinese medicine).Methods Ovariectomized rats as climacteric syndrome model were used to study the effects of HRG on serum estradiol level by radio-immunoassay and on hypothalamic monoamine neurotransmitters contents by high performance liquid chromatography and electrochemical detector.Adrenal gland index and thymus index were also determined.Results It was found that HRG could increase the contents of serum estradiol in OVX rats.HRG could reduce the contents of hypothalamic 5-HT and 5-HIAA,which were increased in OVX rats.And it could increase the contents of NE and DA of hypothalamus in OVX rats.HRG could also improve the function of immunity.Conclusions HRG can produce effects on declined neuroendocrine-immune network in OVX rats from various levels,ways and links by means of regulating the function of plant nerve and reproduction endocrine and immunity.So it can stabilize inner condition of body and relieve symptoms of climacteric syndrome.

关键词(KeyWords)： 更年期综合征;红人归胶囊;去势大鼠;神经内分泌免疫调节
climacteric syndrome;Hongrengui capsule;ovariectomized rat;neuroendocrine-immune regulation

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 王芳,张亚宏,左代英,吴英良
WANG Fang1,ZHANG Ya-hong2,ZUO Dai-ying2,WU Ying-liang2(1.Library

参考文献(References)：

• [1]叶雪清.围绝经期神经内分泌免疫网络的变化[J].实用妇产科杂志,1997,13(2):59-60.
• [2]徐淑云,卞如濂,陈修.药理实验方法学[M].北京:人民卫生出版社,2002:1538-1539.
• [3]翟琪.知母总苷对氢考动物植物神经内分泌免疫及应激的调节作用[D].沈阳:沈阳药科大学,2005:16-17.
• [4]李仪奎.中药药理实验方法学[M].上海:上海科学技术出版社,1991:155-156.
• [5]张新民,沈自尹.补益中药对老龄雄性大鼠下丘脑神经递质-甲状腺轴机能作用的研究[J].上海医科大学学报,1990,17(5):365-369.
• [6]SHUGHRUE P J,MERCHENTHALER I.Evidencefor novel estrogen binding sites in the rat hippocampus[J].Neuro-Science,2000,1999(4):605-612.
• [7]李大金,李超荆.中药复方对更年期综合征妇女内分泌-免疫功能的调节[J].上海免疫学杂志,1995,15(5):257-260.
• [8]王滨,刘宏艳,王红.更年乐对更年期综合征网络机制影响的实验研究[J].江苏中医药,2002,23(10):56-57.
• [9]ENMARK E,PELTO-HUIKKO M,GRANDIEN K,et al.Human estrogen receptor gene structure,chromo-somal localization,and expression pattern[J].Clin En-docrinol Metab,1997,82:4258-4265.
• [10]李大金,李超荆,俞瑾,等.更年期综合征生殖内分泌-免疫功能[J].上海医学,1996,19(1):42-44.
• [11]叶雪清.更年期综合征-神经内分泌免疫网络的变化[M].西安:陕西科学技术出版社,1996:171.