李民;苏兴仁;
Li Min 202 Hospital of PLA, Shenyang Su Xingren Shenyang College of Pharmacy

• 1: 中国人民解放军二○二医院
• 2:沈阳药学院药理教研室

摘要(Abstract)：

以放射自显影的方法研究了鸦胆子油乳剂对小鼠艾氏腹水癌细胞的杀伤动力学。³H—TdR标记后0.5小时腹腔注射鸦胆子油乳剂0.2mg/只,损伤的标记细胞在给药后8小时为22.4％,损伤的非标记细胞为8.5％,零时的标记指数为24％,到24小时下降至5.2％,给药后1—4小时有丝分裂指数亦有下降。用秋水仙酰胺阻断法观察,发现该药可以抑制S期细胞进入M期。药物杀伤结合秋水仙酰胺抑制实验证明,给药时正处于M期的细胞仍可继续完成分裂,而G_2期细胞进入M期明显受到抑制。连续18小时腹腔注射3H—TdR标记后0.5小时腹腔注射鸦胆子油乳剂0.2mg/只,损伤的标记细胞在给药后8小时为22.4％,损伤的非标记细胞为8.5％,零时的标记指数为24％,到24小时下降至5.2％,给药后1—4小时有丝分裂指数亦有下降。用秋水仙酰胺阻断法观察,发现该药可以抑制S期细胞进入M期。药物杀伤结合秋水仙酰胺抑制实验证明,给药时正处于M期的细胞仍可继续完成分裂,而G_2期细胞进入M期明显受到抑制。连续18小时腹腔注射³H—TdR后,未标记的G。期细胞的损伤达17.0％。体内实验证明,该药对3H—TdR参入艾氏腹水癌细胞DNA合成具有明显抑制。根据以上结果认为鸦胆子油乳剂可能属于细胞周期非特异性药物。
The cytokinetic effects of intravenous emulsion of seminal oil of Brucea javaniea (IESOBJ) on Ehrlich ascitic carcinoma (EAC) cells were studied autoradiographically. EAC cells of mice were labeled with 3H—TdR后,未标记的G。期细胞的损伤达17.0％。体内实验证明,该药对3H—TdR参入艾氏腹水癌细胞DNA合成具有明显抑制。根据以上结果认为鸦胆子油乳剂可能属于细胞周期非特异性药物。
The cytokinetic effects of intravenous emulsion of seminal oil of Brucea javaniea (IESOBJ) on Ehrlich ascitic carcinoma (EAC) cells were studied autoradiographically. EAC cells of mice were labeled with ³H—TdR. half an hour later 0.2mg of IESOBJ was injected into the peritoneal space of each mouse. 8 hours after administration, the injured cells which had been labelled were 22.4%, and the injured cells which had not been labelled were 8.5%. The labelling index at the zero time, were 24%, 24 hours later it was diminished to 5.2%, 1—4 hours after the administration the mitotic index also dropped. By meams of the colcemide blocking technique,it was found out that the drug prevented the cells of S phase from passing into the M phase. Drug killing together with colcemide suppressing experiment confirmed that cells already in the M phase at the time of injection could be gone completing mitosis while the cells of G_2 phase were markedly suppressed in progressing into the M phase. Afterr repeated peritoneal injection of 3H—TdR. half an hour later 0.2mg of IESOBJ was injected into the peritoneal space of each mouse. 8 hours after administration, the injured cells which had been labelled were 22.4%, and the injured cells which had not been labelled were 8.5%. The labelling index at the zero time, were 24%, 24 hours later it was diminished to 5.2%, 1—4 hours after the administration the mitotic index also dropped. By meams of the colcemide blocking technique,it was found out that the drug prevented the cells of S phase from passing into the M phase. Drug killing together with colcemide suppressing experiment confirmed that cells already in the M phase at the time of injection could be gone completing mitosis while the cells of G_2 phase were markedly suppressed in progressing into the M phase. Afterr repeated peritoneal injection of ³H—TdR,for 18 hours unlabelling G_0 phase cells were 47.8%. On this condition IESOBJ was administered. Five hours later the damaged cells of G_0 phase were about 17.0%.In vivo experiment confirmed that the drug markedly inhibit the 3H—TdR,for 18 hours unlabelling G_0 phase cells were 47.8%. On this condition IESOBJ was administered. Five hours later the damaged cells of G_0 phase were about 17.0%.In vivo experiment confirmed that the drug markedly inhibit the ³H—TdR from incorporating into the EAC to synthesize the DNA. Based on the above description, the conclusion can be reached that IESOBJ may belong to the category of cycle nonspecific agents.

关键词(KeyWords)： 细胞杀伤动力学;艾氏腹水癌;有丝分裂指数;秋水仙酰胺;去氧核糖核酸
Cytokinetic effect; Ehrlich ascitic carcinoma (EAC); Mitotic index; Colcemide; DNA

Abstract:

Keywords:

基金项目(Foundation):

作者(Authors): 李民;苏兴仁;
Li Min 202 Hospital of PLA, Shenyang Su Xingren Shenyang College of Pharmacy

参考文献(References)：

• 1． Lee PS, Chin Med J 68: 103, 1950
• 2． 苏兴仁等:沈阳药学院学报 总11期:15,1979
• 3． 苏兴仁,李民等:中草药 4:21,1981
• 4． 苏兴仁、李民等:沈阳药学院学报 总14期:8,1981
• 5． 潘震昆,韩锐:生物化学与生物物理学报 12:No.1,1980
• 6． Yankee RA, et al: Cancer Res 27:2381, 1976
• 7． 李民等:药学通报 17:631,1982