马桂蕾,黄海华,孙玉明,钟大放
MA Gui-lei1; HUANG Hai-hua1; SUN Yu-ming2; ZHONG Da-fang2(1. School of Pharmaceutical Engineering;

• 1:沈阳药科大学制药工程学院
• 2:沈阳药科大学制药工程学院
• 3:沈阳药科大学药物代谢与药物动力学实验室
• 4:沈阳药科大学药物代谢与药物动力学实验室 辽宁沈阳110016

摘要(Abstract)：

目的以微生物为模型 ,对柳胺酚的二相药物代谢产物进行研究。方法选用 7株丝状真菌为转化菌株 ,利用液相色谱 质谱联用技术对药物代谢产物进行检测。结果柳胺酚经过所选真菌转化后 ,形成葡萄糖结合物和五碳糖结合物 2种二相药物代谢结合产物 ,其中葡萄糖结合物为主要产物 ,产率最高可达 83 %左右 ;且无一相药物代谢产物形成。结论利用特定的微生物可建立研究柳胺酚二相代谢适宜的体外模型。
Objective To study phase Ⅱ metabolites of osalmide in the microbial model. Method Metabolites produced by 7 filamentous fungi screened were identified by liquid chromatography-tandem mass spectrometry (LC-MSn). Result Transformed by 7 fungi screened, the osalmide was converted to two phase Ⅱ metabolites:glucosides and pentosides,in which glucosides were the major product with the highest yield of 83%. In addition,no phase Ⅰ metabolites were produced. Conclusion The specific microorganism may serve as useful models for the study on phase Ⅱ metabolism of the osalmide.

关键词(KeyWords)： 柳胺酚;微生物转化;二相药物代谢产物;小克银汉霉菌;液相色谱串联质谱分析
osalmide; microbial transformation; phase Ⅱ metabolites; Cunninghamella species; liquid chromatography-tandem mass spectrometry

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目 ( 3 993 0 1 80 )

作者(Authors): 马桂蕾,黄海华,孙玉明,钟大放
MA Gui-lei1; HUANG Hai-hua1; SUN Yu-ming2; ZHONG Da-fang2(1. School of Pharmaceutical Engineering;

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