关潇滢;王铁杰;李军;陈晓辉;毕开顺;
GUAN Xiao-ying1,WANG Tie-jie2,LI Jun2,CHEN Xiao-hui1,BI Kai-shun1(1.School of Pharmacy,Shenyang Pharmaceutical University,Shenyang 110016,China;2.Shenzhen Institute For Drug Control,Shenzhen 518029,China)

• 1:沈阳药科大学药学院
• 2:深圳市药品检验所

摘要(Abstract)：

目的建立同时测定大株红景天中红景天苷、酪醇和没食子酸含量的方法。方法采用HPLC法。色谱柱为Inertsil ODS-SP柱(250 mm×4.6 mm,5μm);流动相为乙腈-体积分数0.03%磷酸水溶液(体积比7∶93);检测波长为220 nm;流速为1.0 mL.min-1。结果红景天苷、酪醇和没食子酸质量浓度分别在4.100¹21.9 mg.L-1(r=0.999 9,n=6)、1.10121.9 mg.L-1(r=0.999 9,n=6)、1.10³1.9 mg.L-1(r=0.999 8,n=6)、2.0031.9 mg.L-1(r=0.999 8,n=6)、2.00⁶0.7 mg.L-1(r=0.999 9,n=6)内与峰面积呈良好的线性关系。平均回收率(n=9)分别为99.1%、98.9%和103.0%,RSD分别为3.0%、2.1%和0.8%。结论本方法快捷、简便,结果准确、可靠、重复性好,可作为大株红景天药材中红景天苷、酪醇和没食子酸的测定方法。
Objective To establish a method for the determination of salidroside,tyrosol and gallic acid in Rhodiola kirilowii(Regel) Maxim by HPLC.Methods The chromatographic method was carried out on Inertsil ODS-SP column(250 mm×4.6 mm,5 μm) using acetonitrile-0.03% phosphoric acid(V∶V=7∶93) as the mobile phase;the detection wavelength was set at 220 nm and the flow rate was 1.0 mL·min-1.Results The linear rangers were 4.100-121.9 mg·L-1 for salidroside,1.10-31.9 mg·L-1 for tyrosol and 2.00-60.7 mg·L-1 for gallic acid,respectively.The average recoveries(n=9) were 99.1%,98.9% and 103.0%;RSD(n=9) were 3.0%,2.1% and 0.8%.Conclusions The method is simple,accurate with good reproducibility.It can be used for the quality control of Rhodiola kirilowii(Regel) Maxim.

关键词(KeyWords)： 大株红景天;红景天苷;酪醇;没食子酸;高效液相色谱法
Rhodiola crenulate;salidroside;tyrosol;gallic acid;HPLC

Abstract:

Keywords:

基金项目(Foundation):

作者(Authors): 关潇滢;王铁杰;李军;陈晓辉;毕开顺;
GUAN Xiao-ying1,WANG Tie-jie2,LI Jun2,CHEN Xiao-hui1,BI Kai-shun1(1.School of Pharmacy,Shenyang Pharmaceutical University,Shenyang 110016,China;2.Shenzhen Institute For Drug Control,Shenzhen 518029,China)

DOI: 10.14066/j.cnki.cn21-1349/r.2010.05.007

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