杨艳燕,李顺意,高尚,闫达中,陈勇
YANG Yan yan; LI Shun yi; GAO Shang; YAN Da zhong; CHEN Yong (School of Life Sciences;

• 1:湖北大学生命科学学院
• 2:湖北大学生命科学学院
• 3:湖北大学生命科学学院
• 4:湖北大学生命科学学院

摘要(Abstract)：

利用改良的培养基 纤维蛋白平板法 ,从我国传统食品豆豉中筛选出具有高纤溶活性的纳豆杆菌菌株 ;菌株液体发酵法制得粗酶液 ,采用沉淀法及柱层析等方法从发酵液中初步分离纳豆激酶 ;并应用体外溶栓实验考察分析了纳豆激酶的纤溶活性 ,为开发新型溶栓药物及保健食品提供了实验参考依据
Two Bacillus subtilis strains with high fibrinolysis activity were screened from traditional Chinese food Douchi with an improved medium fibrinogen plate. A study and optimization on the fermentation process, nattokinase has been separated from the fermented fluid with precipitation and column chromatography. The fibrinolysis activity of nattokinase was determined and analyzed in the thrombolysis experiment in vitro . The method will provide experimental evidence for developing new thrombolytic medicine and health food.

关键词(KeyWords)： 纳豆杆菌;纳豆激酶;纤溶活性
Bacillus subtilis ; nattokinase; fibrinolysis activity

Abstract:

Keywords:

基金项目(Foundation): 湖北省教育厅基金资助项目 ( 99D0 0 3) ;; 湖北省生物化学与分子生物学重点学科基金资助

作者(Authors): 杨艳燕,李顺意,高尚,闫达中,陈勇
YANG Yan yan; LI Shun yi; GAO Shang; YAN Da zhong; CHEN Yong (School of Life Sciences;

参考文献(References)：

• [1]SumiH ,HamadaH ,TsushimaH ,etal.Anovelfibri nolyticenzymeinthevegetablecheesenatto:atypicalandpopularsoybeanfoodintheJapanesediet[J].Ex perientia,1987,43(20):1110-1111.
• [2]王正刚,丁贵平,蔡正森.纳豆激酶的研究进展[J].中国生化药物杂志,1998,19(6):401-403.
• [3]李荣萍,李晶,赵晓祥,等.一种具有纤溶活性的枯草杆菌蛋白激酶的研究[J].生物技术,1996,6(3):21-23.
• [4]高尚,杨艳燕,马立新,等.纳豆杆菌的筛选[J].湖北大学学报(自然科学版),2000,22(专辑):184-185.
• [5]徐涛,宋文延.一种新型纤溶活性物质———纳豆激酶[J].中国生化药物杂志,1995,16(4):196.
• [6]FujitaM ,HongK ,ItoY .TransportofNKacrossoftheratintestinaltract[J].BiolPharmBull,1995,18(9):1194-1196.
• [7]SumiH ,HamadaH ,NakanishiK .Enhancementofthefibrinolyticactivityinplasmabyoraladministrationofnattokinase[J].ActaHaematol,1990,84(1):139-143.