毛细管电泳溶质系统的热力学研究Study on thermodynamic variation of solutes in capillary electrophoresis
孙国祥,孙毓庆
SUN Guo-xiang; SUN Yu-qing (School of Pharmacy;
摘要(Abstract)：
目的对毛细管区带电泳 (CZE)分离体系中溶质系统的能量变化进行研究。方法采用热力学方法。结果对CZE的溶质系统提出了溶质分离自由能ΔGsep、混合自由能ΔGmix、整体动能增量ΔT、整体势能增量ΔU和溶质能量净增值ΔE ,以及表观作功时间teff、分离功份额τ和单位峰能耗W0 等概念 ,并以实验研究了各项的变化规律。结论当背景电解质 (BGE)浓度和电压增大时 ,W0 增大 ;但teff和τ呈降低变化。ΔT随BGE浓度升高而降低 ,随电压增大而增大 ;ΔGsep和ΔU为恒定值。溶质系统的能量净增值ΔE主要由ΔGsep和ΔGmix决定 ,ΔT和ΔU远远小于ΔGsep,可忽略
Objective To study the enegy changing rules of solutes in the capillary zone electrophoresis(CZE)Methods The thermodynamic theory method was used.Results A series of energy concepts about the solutes in CZE has been set up ,in which the separation free energy ΔG sep, mixed free energy ΔG mix, total kinetic energy variationΔT, total potential energy variationΔU, net increasing energy of solutes ΔE, apparent working time t eff, exhausted energy per peak W 0 and share of the net increasing energy were all included. The influences of the concentration of background electrolytes(BGE)and voltage applied on them were investigated by experiments.Conclusions W 0 increased with the increasing of the concentration of BGE or voltage, but τand t eff decreased.ΔT decreased as BGE concentration was increased,but increased as the voltage was applied highly. Under both conditions, ΔG sep and ΔU remained stable. Because ΔT and ΔU were much less than ΔE , they almost could be ignored. The separation free energy ΔG sep and mixed free energyΔG mix played important roles in contribution to the net increasing energy of solutes. If we could have gained a better separation in CE, we should have selected both approptiate BGE concentration and appropriate voltage to performe CE experiments.
关键词(KeyWords)：
毛细管区带电泳;分离自由能ΔGsep;混合自由能ΔGmix;溶质能量净增值ΔE
CZE;separation free energy ΔG sep; mixed free energy ΔG mix; net increasing energy of solutes ΔE
基金项目(Foundation):
作者(Authors):
孙国祥,孙毓庆
SUN Guo-xiang; SUN Yu-qing (School of Pharmacy;