程卓阳,周乐,刘思帆,白明,宋少江,黄肖霄
CHENG Zhuo-yang,ZHOU Le,LIU Si-fan,BAI Ming,SONG Shao-jiang,HUANG Xiao-xiao

• 1:沈阳药科大学教育部"基于靶点的创新药物研究与设计重点实验室"

摘要(Abstract)：

目的为寻找新的抗氧化剂来源,对蔷薇科山楂属植物山楂(Crataegus pinnatifida Bge.)核的体积分数70%乙醇提取物进行化学成分及其抗氧化活性的研究。方法运用D101大孔树脂、硅胶柱色谱、ODS柱色谱、制备HPLC等手段进行化学成分的分离纯化,根据理化性质及波谱数据鉴定其结构,并对分离得到的化合物进行DPPH和ABTS自由基清除抗氧化活性筛选实验研究。结果从山楂核体积分数为70%的乙醇水溶液的提取物中分离得到4个芳香族化合物,分别鉴定为threo-7R,8R-7-O-ethylguaiacylglycerol(1),erythro-7R,8S-7-O-ethylguaiacylglycerol(2),2,4-dimethoxyphenyl-1-O-β-D-glucopyranoside(3),vanillic acid-β-D-glucopyranoside(4)。结论化合物1和2为2个绝对构型未见报道的新化合物,化合物3和4为首次从山楂属植物中分离得到。同时,抗氧化活性结果显示化合物1和2具有较强的抗氧化活性。
Objective To study the chemical constituents of 70% ethanol extraction from the seeds of Crataegus pinnatifida and their antioxidant activities for the research of newsources of antioxidant compounds.Methods Four compounds were isolated with D101 macro porous resin,silica gel,ODS,and preparation HPLC,etc. On the basis of analysis of NM R,M S data and measurement of physicochemical characters,the chemical structures of the four compounds were elucidated. In addition,antioxidant activities of these compounds were evaluated using 2,2-diphenyl-1-pikrylhydrazyl( DPPH) and 2,2'-azino-bis( 3-ethylbenzothiazoline-6-sulphonic acid)( ABTS) assays. Results Four aromatic compounds were isolated from 70% ethanol extraction and their structures were identified as threo-7R,8R-7-O-ethylguaiacylglycerol( 1),erythro-7R,8S-7-O-ethylguaiacylglycerol( 2),2,4-dimethoxyphenyl-1-O-β-D-glucopyranoside( 3) and vanillic acid-β-Dglucopyranoside( 4). Conclusions Compounds 1 and 2 are newcompounds; compounds 3 and 4 are isolated from the genus Crataegus for the first time. Compounds 1 and 2 showpotent DPPH and ABTS radical scavenging activity.

关键词(KeyWords)： 山楂核;化学成分;结构鉴定;抗氧化活性
seeds of Crataegus pinnatifida;chemical constituents;structural identification;antioxidant activities

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 程卓阳,周乐,刘思帆,白明,宋少江,黄肖霄
CHENG Zhuo-yang,ZHOU Le,LIU Si-fan,BAI Ming,SONG Shao-jiang,HUANG Xiao-xiao

DOI: 10.14066/j.cnki.cn21-1349/r.2015.11.003

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