彭缨,骆昉,王淑君,李玲芝,孙毓庆,潘卫三
PENG Ying,LUO Fang,WANG Shu-jun,LI Ling-zhi,SUN Yu-qing,PAN Wei-san(School of Pharmacy

• 1:沈阳药科大学药学院
• 2:沈阳药科大学药学院
• 3:沈阳药科大学药学院
• 4:沈阳药科大学药学院

摘要(Abstract)：

目的建立用苯甲酰氯与菝葜皂苷元进行柱前衍生化反应,以HPLC-UV法测定知母药材中菝葜皂苷元的含量。方法采用Agilent C18柱(150 mm×4.6 mm,5μm),流动相为甲醇-水(体积比99∶1),检测波长为230 nm,流速为1.1 mL.min-1,柱温为33℃。结果菝葜皂苷元质量浓度在0.20~4.00 g.L-1内与峰面积呈良好的线性关系(r=0.999 6),平均回收率为98.3%(n=9),RSD为2.1%。结论本法可用于菝葜皂苷元的定量分析。
Objective To establish an HPLC-UV method for determination of sarsasapogenin in Rhizoma Anemarrhenae with benzoyl chloride derivate.Methods Chromatography was carried out on Agilent C18 column(150 mm×4.6 mm,5 μm),using methanol-water(V∶V=99∶1) as the mobile phase at a flow rate of 1.1 mL·min-1 and the detection wavelength was set at 230 nm.The column temperature was 33 ℃.Results The linear range of sarsasapogenin was 0.20-4.00 g·L-1(r=0.999 6).The average recoveries of sarsasapogenin was 98.3%(n=9).Conclusions This method can be used for determination of sarsasapogenin in Rhizoma Anemarrhenae.

关键词(KeyWords)： 知母;菝葜皂苷元;柱前衍生化;高效液相色谱法
Rhizoma Anemarrhenae;sarsasapogenin;precolumn derivatization;HPLC

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 彭缨,骆昉,王淑君,李玲芝,孙毓庆,潘卫三
PENG Ying,LUO Fang,WANG Shu-jun,LI Ling-zhi,SUN Yu-qing,PAN Wei-san(School of Pharmacy

参考文献(References)：

• [1]国家药典委员会.中华人民共和国药典:一部[M].北京:化学工业出版社,2005:148.
• [2]季德,陆兔林,宋.知母研究进展[J].中华实用中西医杂志,2005,18(13):260-262.
• [3]于健东,马玲云,马双成,等.知母中知母皂苷BⅡ的TLC鉴别及HPLC法含量测定[J].药物分析杂志,2005,25(12):1436-1437.
• [4]刘美凤,丁怡,邢东明,等.RP-HPLC示差折光检测法测定复方知母胶囊中菝葜皂苷元的含量[J].中国药学杂志,2004,39(2):141-142.