HPLC-ELSD法测定人参不同部位13种人参皂苷的含量Quantitative determination of thirteen kinds of ginsenoside in different part of panax ginseng by HPLC-ELSD
冯素香,李先贺,李蒙蒙,王哲,张蕾,高宁宁
FENG Su-xiang,LI Xian-he,LI Meng-meng,WANG Zhe,ZHANG Lei,GAO Ningning
摘要(Abstract)：
目的建立同时测定人参药材主根、侧根、须根、芦头中13种人参皂苷类成分含量的高效液相色谱-蒸发光散射检测器(high performance liquid chromatography-evaporative light scattering detector,HPLC-ELSD)法。方法采用Venusil XBP C_(18)(L)色谱柱(250 mm×4.6 mm,5μm),流动相:乙腈-水梯度洗脱,流速:1 mL·min~(-1),柱温:20℃,蒸发光散射检测器参数:载气流量为2.9 L·min~(-1),压力为5.5×10~5Pa,漂移管温度为107℃,增益为1。结果人参皂苷Rg_1、Re、Rf、Rh_1、Rg_2、Rb_1、Rc、Rb_2、Rb_3、Rd、F_2、Rh_4、Rg_3分别在0.90~18.04、0.93~18.50、0.77~15.40、0.30~6.02、0.30~6.05、1.80~36.00、1.31~26.26、1.37~27.40、0.30~6.05、0.52~10.33、0.30~6.12、0.30~6.02、0.30~6.02μg内呈良好的线性关系(r>0.999 0),平均加样回收率(n=6)为97.1%~101.8%,RSD≤3.0%。结论该方法可用于同时测定13种人参皂苷的含量。
Objective To develop an HPLC-ELSD method for simultaneous determination the content of thirteen kinds of ginsenoside in taproot, lateral root,fibrous roots or rhizoma of the ginseng medicinal herbs.Methods Venusil XBP C_(18)( L) column was used to separate the components with a mobile phase of acetonitrile-water by gradient elution. The flow rate was 1. 0 mL·min~(-1),and the column temperature was20 ℃. For ELSD, the carrier gas flow rate was set at 2. 9 L·min~(-1),gas pressure at 5. 5 × 10~5 Pa, the drift tube temperature at 107 ℃ and the gain was set at 1. Results Good linearity( r > 0. 999 0) was obtained in the range of 0. 90-18. 04,0. 93-18. 50,0. 77-15. 40,0. 30-6. 02,0. 30-6. 05,1. 80-36. 0,1. 31-26. 26, 1. 37-27. 40, 0. 30-6. 05, 0. 52-10. 33, 0. 30-6. 12, 0. 30-6. 02, 0. 30-6. 02 μg for ginsenoside Rg_1,Re,Rf,Rh_1,Rg_2,Rb_1,Rc,Rb_2,Rb_3,Rd,F_2,Rh_4,Rg_3, respectively. The average recovery(n=6) was between 97. 1%-101. 8%,and the RSD≤3. 0%. Conclusions This method can be used to determine the content of thirteen kinds of ginsenosides. It has the advantages of high separation efficiency,good repeatability and accuracy,and simple operation process.
关键词(KeyWords)：
人参皂苷;高效液相色谱法;蒸发光散射检测器;含量测定
ginsenoside;HPLC;ELSD;content determination
基金项目(Foundation): 国家自然科学基金资助项目(81130062)
作者(Author):
冯素香,李先贺,李蒙蒙,王哲,张蕾,高宁宁
FENG Su-xiang,LI Xian-he,LI Meng-meng,WANG Zhe,ZHANG Lei,GAO Ningning
DOI: 10.14066/j.cnki.cn21-1349/r.2017.07.006
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