栾爽,董振敏,赵怀清,申涛,高靥,马丽丽
LUAN Shuang1,DONG Zhen-min2,ZHAO Huai-qing1,SHEN Tao1,GAO Ye1,MA Li-li1(1.School of Pharmacy

• 1:沈阳药科大学药学院
• 2:辽河油田卫生事业管理部

摘要(Abstract)：

目的建立HPLC法测定心脑联通胶囊中丹酚酸B和大豆苷元含量的方法。方法采用HPLC法。Diamonsil ODS柱(200 mm×4.6 mm,5μm),以甲醇-体积分数为0.1%磷酸水溶液(体积比为41.5∶58.5)为流动相,流速为1.0 mL.min-1,柱温为35℃,检测波长为280 nm。结果丹酚酸B质量浓度在19.2~153.6 mg.L-1内、大豆苷元质量浓度在2.8~22.4 mg.L-1内与色谱峰面积呈良好的线性关系,相关系数分别为0.999 5和0.999 7。方法平均回收率分别为100.8%(RSD=1.0%)和99.2%(RSD=1.1%)。结论本方法操作简便,测定结果准确可靠,可用于心脑联通胶囊的质量控制。
Objective To develop a quantitative method for determination of salvianolic acid B and daidzein in Xinnaoliantong(traditional Chinese medicine)capsules by HPLC.Methods Diamonsil ODS column(200 mm×4.6 mm,5 μm) was used.The mobile phase was composed of methanol-0.1% H3PO4(V∶V=41.5∶58.5).The flow rate was 1.0 mL·min-1 and column temperature was 35 ℃.The detective wavelength was set at 280 nm.Results The calibration cure was linear within the range of 19.2-153.6 mg·L-1(r=0.999 5) and 2.8-22.4 mg·L-1(r=0.999 7) for salvianolic acid B and daidzein,respectively;the average recoveries were 100.8%(RSD=1.0%) and 99.2%(RSD=1.1%) for salvianolic acid B and daidzein,respectively.Conclusions The method is sensitive,accurate and can be used for the quality control of Xinnaoliantong capsules.

关键词(KeyWords)： 心脑联通胶囊;高效液相色谱法;丹酚酸B;大豆苷元
Xinnaoliantong capsule;HPLC;salvianolic acid B;daidzein

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 栾爽,董振敏,赵怀清,申涛,高靥,马丽丽
LUAN Shuang1,DONG Zhen-min2,ZHAO Huai-qing1,SHEN Tao1,GAO Ye1,MA Li-li1(1.School of Pharmacy

参考文献(References)：

• [1]中国医学科学院药物研究所.中草药现代研究:第二卷[M].北京:北京医科大学中国协和医科大学联合出版社,1996:472.
• [2]章育中,杨凡.高效液相色谱法测定葛根及其片剂中异黄酮的含量[J].药物分析杂志,1984,4(2):67.
• [3]康阿龙,汤迎爽,王天,等.RP-HPLC测定乙肝病毒清颗粒中丹酚酸B的含量[J].中成药,2006,28(12):1860-1861.