高效液相色谱法测定麝香抗栓丸中芍药苷的含量HPLC determination of the content of paeoniflorin in Shexiang Kangshuan Pills
张莉,哈永红,梁春华,沙英威
ZHANG Li; HA Yong hong; LIANG Chun hua; SHA Ying wei (Jilin Institute for Drug Control;
摘要(Abstract)：
目的建立HPLC测定麝香抗栓丸中芍药苷的含量。方法以芍药苷为对照品 ,乙腈 0 1 %磷酸溶液 ( 1 5∶85 ,mL/mL)为流动相 ,KromasilC18ODS色谱柱 ( 1 0 μm,2 5 0mm× 4 6mm)为固定相 ,流速为 0 8mL/min,检测波长为 2 30nm ;柱温为室温。结果芍药苷在 0 2 0～ 0 82mg/mL线性良好 ,r值为 0 9997,平均回收率 (x±RSD) %为 ( 1 0 0 5± 1 5 ) %。结论此法可较好地用于麝香抗栓丸的质量控制。
Objective A HPLC method was used to determine the content of paeoniflorin in Shexiang Kangshuan Pills.Methods The method was to use paeoniflorin as the standard. The method was carried out by using the Kromasil C 18 ODS column and acetonitrile 0 1%phosphoric acid(15∶85,mL/mL)as the mobile phase. The UV absorbency was monitored at 230 nm .Flow rate was 0 8 mL/min . The column temperature was room temperature. Results A better linearity and good correlation coefficient were obtained from 0 20 to 0 82 mg/mL ( r =0 999 7 ).Conclusion The mean recoveries and RSD were (100 5±1 5)%.The method is accurate, rapid and simple.
关键词(KeyWords)：
麝香抗栓丸;芍药苷;高效液相色谱
Shexiang Kangshuan Pills; paeoniflorin;HPLC
基金项目(Foundation):
作者(Authors):
张莉,哈永红,梁春华,沙英威
ZHANG Li; HA Yong hong; LIANG Chun hua; SHA Ying wei (Jilin Institute for Drug Control;