复方鸦胆子油亚微乳注射液中油酸在大鼠体内的药物动力学Pharmacokinetics of oleic acid in compound brucea javanica oil submicron emulsions for injection in rats
于叶玲,唐星,崔福德
YU Ye-ling,TANG Xing,CUI Fu-de(School of Pharmacy
摘要(Abstract)：
目的研究复方鸦胆子油亚微乳静脉给药在大鼠体内的药物动力学特征,并与鸦胆子油和薏苡仁油单方乳剂进行比较。方法大鼠尾静脉注射2 mL.kg-1复方鸦胆子油亚微乳、鸦胆子油乳及薏苡仁油乳,建立柱前衍生化HPLC-UV法测定血浆中的油酸浓度。结果油酸质量浓度在52.08~868.0 mg.L-1内线性良好(r=0.999 2),方法回收率大于95%,日内、日间精密度RSD<15%。采用统计矩的非隔室动力学理论对复方鸦胆子油亚微乳、鸦胆子油乳及薏苡仁油乳的血药数据进行处理,ρmax分别为(1.749±0.240)、(1.568±0.470)和(1.222±0.388)g.L-1,t1/2分别为(23.43±6.13)(、25.56±4.10)和(21.30±7.34)min,AUC分别为(61.2±15.2)、(55.2±12.7)和(41.1±15.1)min.g.L-1。结论方法灵敏度高、准确、可靠。3种乳剂在大鼠体内生物学过程主要以乳滴的分布、消除为主,三者的药物动力学参数之间无显著性差异。
Objective To study the pharmacokinetics of compound brucea javanica oil submicron emulsions,brucea javanica oil emulsions and coix seed oil emulsions in rats.Methods The emulsions were administered through tail vein.A pre-column derivation HPLC-UV method for determination of oleic acid in rat plasma was developed.Results The standard curve was linear over the concentration range of 52.08-868.0mg·L-1(r=0.9992).The method had a high recovery(>95%) with intraday and interday precision less than 15%.The pharmacokinetic parameters of oleic acid were evaluated by noncompartmental method.t1/2 were(23.43±6.13)min,(25.56±4.10)min and(21.30±7.34)min,AUC were(61.2±15.2)min·g·L-1,(55.2 ± 12.7)min·g·L-1 and(41.1±15.1)min·g·L-1,respectively.Conclusions The pre-column derivation HPLC-UV method has characteristics of high sensitivity,accuracy and reliability.There are no significant differences amoung the pharmacokinetic parameters of three emulsions.
关键词(KeyWords)：
鸦胆子油;薏苡仁油;亚微乳;药物动力学
brucea javanica oil;coix seed oil;submicron emulsions;pharmacokinetics
基金项目(Foundation): 国家高技术研究发展计划(863计划)课题(2004AA2z3240)
作者(Author):
于叶玲,唐星,崔福德
YU Ye-ling,TANG Xing,CUI Fu-de(School of Pharmacy
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