多西他赛纳米脂质载体的制备及其性质考察Preparation and characteristics of docetaxel nanos-tructure lipid carriers
杨平,孙进,刘凯,常笛,赵焰平,何仲贵
YANG Ping,SUN Jin,LIU Kai,CHANG Di,ZHAO Yan-ping,HE Zhong-gui(School of Pharmacy
摘要(Abstract)：
目的制备多西他赛纳米脂质载体,并考察其剂型性质及体外释药行为。方法采用薄膜超声法制备多西他赛纳米脂质载体,使用透射电镜观察粒子外观形态,光子相关光谱法测定其粒径和分布,微柱离心法测包封率,透析法测体外释药特性。结果在优化条件下制备的纳米级粒子粒径为(124±16)nm,Zeta电位为-19.57 mV,包封率为95.8%。体外释放符合Weibull释放模型。结论制备的多西他赛纳米脂质载体粒径小,药物包封率高,并可实现药物控释。
Objective To prepare docetaxel nanostructured lipid carriers(docetaxel-NLC) and investigate their pharmaceutical properties and drug releasing behavior in vitro.Methods Docetaxel-NLC was formulated by film-dispersion and ultrasonication method.Transmission electron microscopy(TEM) was employed to study the shape.Photon correlation spectroscopy(PCS) was used to study the diameter.The entrapment efficiency was determined by mini-column centrifugation.The in vitro drug releasing behavior was studied by dialysis.Results The mean diameter of NLC was(124±16) nm with Zeta potential of-19.57 mV,and the mean entrapment efficiency was 95.8%.The in vitro drug release was fitted well with Weibull equation.Conclusions Docetaxel-NLC with small particle sizes can improve drug loading capacity and its controlled releasing properties.
关键词(KeyWords)：
多西他赛;纳米脂质载体;包封率;体外释放
docetaxel;nanostructured lipid carriers;entrapment efficiency;in vitro drug release
基金项目(Foundation): 辽宁省科技厅科技项目(2007402071)
作者(Author):
杨平,孙进,刘凯,常笛,赵焰平,何仲贵
YANG Ping,SUN Jin,LIU Kai,CHANG Di,ZHAO Yan-ping,HE Zhong-gui(School of Pharmacy
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