高靥,王冬梅,栾爽,申涛,李金银,赵怀清
GAO Ye,WANG Dong-mei,LUAN Shuang,SHEN Tao,LI Jin-yin,ZHAO Huai-qing (School of Pharmacy

• 1:沈阳药科大学药学院
• 2:沈阳药科大学药学院
• 3:沈阳药科大学药学院
• 4:沈阳药科大学药学院

摘要(Abstract)：

目的建立同时测定芩连片中盐酸巴马汀和盐酸小檗碱含量的方法,用于芩连片的质量控制。方法采用HPLC法。色谱柱为Diamonsil ODS柱(4.6 mm×200 mm,5μm),流动相为50 mmol.L-1磷酸二氢钠(pH 4.6)-乙腈(体积比72∶28),流速为1.0 mL.min-1,检测波长为343 nm,柱温为35℃。结果芩连片中盐酸巴马汀、盐酸小檗碱与其他成分分离良好;盐酸巴马汀的质量浓度在5.2~52.0 mg.L-1(r=0.9998)内、盐酸小檗碱的质量浓度在30.4~304.0 mg.L-1(r=0.9997)内与峰面积呈良好的线性关系;盐酸巴马汀、盐酸小檗碱的回收率分别为100.1%、99.9%,RSD分别为1.1%、0.80%。结论所建立的同时测定芩连片中盐酸巴马汀和盐酸小檗碱含量的HPLC法准确、重现性好,可用于芩连片的质量控制。
Objective To establish an HPLC method for simultaneous determination of palmatine hydrochloride and berberine hydrochloride in Qinlian tablets.Methods The separation was performed on a Diamonsil C18(4.6 mm ×200 mm,5 μm)column.The mobile phase was composed of 50 mmol·L-1 NaH2PO4(pH 4.6) and acetonitrile at the rate of V∶V=72∶28.The column temperature was set at 35 ℃ and the flow rate was 1.0 mL·min-1,with UV detection at 343 nm.Results The numbers of theoretical plates calculated by palmatine hydrochloride and berberine hydrochloride were 4 000 and 6 500,respectively.The regression equation of palmatine hydrochloride was A=3.706×104ρ+7.369×102(r=0.999 8),which was linear over the ranger of 5.2-52.0 mg·L-1.The regression equation of berberine hydrochloride was A=3.377×104ρ+8.978×103(r=0.999 7),which was linear over the ranger of 30.4-304.0 mg·L-1.The average recovery was 100.1 %(RSD=1.1 %) for palmatine hydrochloride,99.9%(RSD=0.80%) for berberine hydrochloride.Conclusions The method can be used in the quality control of Qinlian tablets.

关键词(KeyWords)： 芩连片;高效液相色谱法;盐酸巴马汀;盐酸小檗碱
Qinlian tablet;HPLC;palmatine hydrochloride;berberine hydrochloride

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 高靥,王冬梅,栾爽,申涛,李金银,赵怀清
GAO Ye,WANG Dong-mei,LUAN Shuang,SHEN Tao,LI Jin-yin,ZHAO Huai-qing (School of Pharmacy

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