GC法测定芫荽茎叶中4种挥发性成分的含量Determination of four volatile components in leaf of Coriandrum sativum L. by GC
姜振元,李清,赵龙山,郑晓娇,陈晓辉,毕开顺
JIANG Zhen-yuan,LI Qing,ZHAO Long-shan,ZHENG Xiao-jiao,CHEN Xiao-hui,BI Kai-shun(School of Pharmacy
摘要(Abstract)：
目的建立一种芫荽茎叶中芳樟醇、癸醛、正癸醇和十一醛的GC含量测定方法。方法以挥发油提取器蒸馏制备供试液;HP-5MSI毛细管色谱柱(30 m×0.25 mm,0.25μm),以氮气为载气,FID检测器,采用程序升温,内标法测定(柠檬烯为内标物)含量。结果芳樟醇、癸醛、正癸醇和十一醛进样质量浓度分别在31.5～315.0 mg.L-1(r=0.999 6)、269.0～2 690.0 mg.L-1(r=0.999 7)、95.3～953.0 mg.L-1(r=0.999 6)、58.1～581.0 mg.L-1(r=0.999 7)内呈良好线性关系,平均回收率分别为102.0(RSD=1.5%)、102.0(RSD=0.6%)、101.9(RSD=1.1%)、102.6(RSD=0.9%)。结论所建立的方法可用于芫荽茎叶中4种挥发性成分含量测定。
Objective To establish a method for determining the contents of linalool,decanal,n-decanol,undecanal in Coriandrum sativum L.leaf.Methods The sample solution was distilled in vaporizing oil extraction apparatus.With HP-5MSI capillary column(30 m×0.25 mm,0.25 μm),nitrogen as the carrier gas,FID as the detector,the programmed temperature-GC and internal standard(Limonene was used for the internal standard)method were employed to determine the components.Results The results showed that the linear ranges of linalool,decanal,n-decanol and undecanal were 31.50-315.0 mg · L-1(r=0.999 6),269.0-2 690.0 mg · L-1(r=0.999 2),95.3-953.0 mg · L-1(r=0.999 8),58.1-581.0 mg · L-1(r=0.999 7).The average recovery of the method were 102.0%(RSD=1.5%),102.0%(RSD=0.6%),101.9%(RSD=1.1%),102.6%(RSD=0.9%).The average recovery of the method were 102.0(RSD=1.5%),102.0(RSD=0.6%),101.9(RSD=1.1%),102.6(RSD=0.9%).Conclusions The results show that this method is steady,accurate and practical which could be used for the determination of linalool,decanal,n-decanol and undecanal in Coriandrum sativum L.leaf.
关键词(KeyWords)：
芫荽;气相色谱;芳樟醇;癸醛;正癸醇;十一醛;含量测定
Coriandrum sativum L.;GC;linalool;decanal;n-decanol;undecanal;content determination
基金项目(Foundation):
作者(Author):
姜振元,李清,赵龙山,郑晓娇,陈晓辉,毕开顺
JIANG Zhen-yuan,LI Qing,ZHAO Long-shan,ZHENG Xiao-jiao,CHEN Xiao-hui,BI Kai-shun(School of Pharmacy
DOI: 10.14066/j.cnki.cn21-1349/r.2012.12.005
参考文献(References)：
- [1]赵秀玲.芫荽的成分及保健功能的研究进展[J].食品工业科技,2011,32(4):427-433.
- [2]LINCK V M,SILVA A L,FIGUEIRO M,et al.Effects ofinhaled Linalool in anxiety,social interaction and ag-gressive behavior in mice[J].Phytomedicine,2010,17(8/9):679-683.
- [3]MATASYOH J C,MAIYO Z C,NGURE R M,et al.Chemical composition and antimicrobial activity of theessential oil of Coriandrum sativum[J].Food Chemis-try,2009,113(2):526-529.
- [4]国家药典委员会.中华人民共和国药典:一部[M].北京:中国医药科技出版社,2010.
- [5]陆占国,郭红转,李伟.超声波法提取芫荽茎叶精油和成分分析以及消除亚硝酸钠作用[J].化学与黏合,2006,28(4):218-221.
- [6]张京娜,陈霞,杨冬,等.云南玉溪芫荽挥发油成分的GC-MS分析[J].现代中药研究与实践,2009,23(4):24-26.