刘永利,袁浩,李冬梅,冯丽
LIU Yong-li,YUAN Hao,LI Dong-mei,FENG Li(Hebei Provincial Insititute for Drug Control

• 1:河北省药品检验所

摘要(Abstract)：

目的建立同时测定镇咳宁糖浆中盐酸麻黄碱、甘草苷和甘草酸含量的方法。方法色谱柱:Shim-pack VP-ODS柱(4.6 mm×250 mm,5μm);流动相:乙腈-体积分数为0.1%磷酸溶液梯度洗脱;检测波长:205 nm(盐酸麻黄碱)、237 nm(甘草苷与甘草酸);流速:1.0 mL.min-1。结果盐酸麻黄碱、甘草苷、甘草酸质量浓度分别在6.222～414.8 mg.L-1、2.335～116.7 mg.L-1、12.45～415.0 mg.L-1内与峰面积呈良好的线性关系,相关系数均为0.999 9,平均回收率分别为98.92%、103.0%和98.24%。结论本方法操作简便,结果可靠,可作为镇咳宁糖浆的质量控制方法。
Objective To establish a method for the determination of ephedrine hydrochloride,liquiritin and glycyrrhizic acid in Zhenkening syrup(traditional Chinese medicines).Methods The separation was performed on a Shim-pack VP-ODS(4.6 mm×250 mm,5 μm)column with the mobile phase consisting of acetonitrile-0.1% phosphoric acid for gradient elution.Ephedrine hydrochloride,liquiritin and glycyrrhizic acid were determined by dual wavelength of 205 nm and 237 nm.Results The linearities were in the ranges of 6.222-414.8 mg · L-1 for ephedrine hydrochloride,2.335-116.7 mg · L-1 for liquiritin,and 12.45-415.0 mg · L-1 for glycyrrhizic acid,respectively.The average recoveries were 98.92%(RSD=0.90%),103.0%(RSD=0.25%),98.24%(RSD=1.9%),respectively.Conclusions This method is convenient,rapid and accurate.It can be used for quality control of Zhenkening syrup.

关键词(KeyWords)： 镇咳宁糖浆;盐酸麻黄碱;甘草苷;甘草酸;高效液相色谱法;含量测定
Zhenkening syrup;ephedrine hydrochloride;liquiritin;glycyrrhizic acid;HPLC;content determination

Abstract:

Keywords:

基金项目(Foundation): 河北省自然科学基金-石药集团医药联合研究基金资助项目(C2011320007)

作者(Author): 刘永利,袁浩,李冬梅,冯丽
LIU Yong-li,YUAN Hao,LI Dong-mei,FENG Li(Hebei Provincial Insititute for Drug Control

DOI: 10.14066/j.cnki.cn21-1349/r.2012.01.005

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