于霞,吴娟,姜雪,王芬,王淼,赵春杰
YU Xia,WU Juan,JIANG Xue,WANG Fen,WANG Miao,ZHAO Chun-jie

• 1:辽宁中医药大学附属第二医院
• 2:沈阳药科大学药学院
• 3:山东中医药大学

摘要(Abstract)：

目的建立同时测定消乳散中甘草苷、橙皮苷和甘草酸铵含量的HPLC法。方法色谱柱为XP Urisil C18(250 mm×4.6 mm,5μm)柱;流动相为乙腈-体积分数为0.1%的磷酸水溶液,梯度洗脱;流速为1.0 m L·min-1;检测波长为237 nm;柱温为35℃。结果甘草苷、橙皮苷和甘草酸铵分别在质量浓度5.20~31.2 mg·L~(-1)、30.0~180 mg·L~(-1)和5.20~31.2 mg·L~(-1)内与峰面积呈良好的线性关系,相关系数r分别为0.999 5、0.999 8和0.999 1,平均回收率分别为99.1%、99.3%和99.2%,RSD分别为1.1%、1.6%和1.3%(n=6)。结论本方法可作为消乳散的质量控制方法。
Objective To establish a method for simultaneous determining the content of liquiritin and hesperidin and ammonium glycyrrhetate in Xiaorusan. Methods The separation was performed on a XP Urisil C18column( 250 mm × 4. 6 mm,5 μm),with acetonitrile-0. 1% phosphoric acid water as mobile phase with gradient elution at flowrate of 1. 0 m L·min-1. The column temperature was set at 35 ℃ and detection wavelength was 237 nm. Results The calibration curves were linear within the range of 5. 20-31. 2 mg·L~(-1)( r = 0. 999 5),30. 0-180 mg·L~(-1)( r = 0. 999 8) and 5. 20-31. 2 mg·L~(-1)( r = 0. 999 1),for liquiritin,hesperidin and ammonium glycyrrhetate respectively. The average recovery rate was 99. 1%,99. 3% and99. 2%( n = 6) and the RSD were 1. 1%,1. 6% and 1. 3%. Conclusions The method can be used as one of the quality control methods for Xiaorusan.

关键词(KeyWords)： 消乳散;甘草苷;橙皮苷;甘草酸铵;含量测定
Xiaorusan;liquiritin;hesperidin;ammonium glycyrrhetate;content determination

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 于霞,吴娟,姜雪,王芬,王淼,赵春杰
YU Xia,WU Juan,JIANG Xue,WANG Fen,WANG Miao,ZHAO Chun-jie

DOI: 10.14066/j.cnki.cn21-1349/r.2017.05.003

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