贾贞,王丹,游松
JIA Zhen,WANG Dan,YOU Song(School of Life Science and Biopharmaceutics

• 1:沈阳药科大学生命科学与生物制药学院

摘要(Abstract)：

目的对一种具有重要生理功能的活性三肽-谷胱甘肽的研究进展作一综述。方法在查阅中外文献的基础上,总结介绍了谷胱甘肽的理化性质和生理功能,重点综述了其制备方法、检测方法和应用情况的最新进展。结果谷胱甘肽在医药和食品工业等领域有着广泛的应用,开发谷胱甘肽具有重大的经济效益和社会效益。结论为谷胱甘肽的进一步研究提供了参考。
Objective To review the progress of glutathione which is a functional non-protein thiol compound.Methods In this summary,the structure,physical properties,chemical properties and physiological functions of glutathione were introduced.The emphasis was focused on the fields of preparation,detection and application of glutathione.Results As a functional antioxidant,glutathione is widely utilized in pharmaceutical and food industry.It will meet the great demand for multiple uses.Conclusions This review provides a reference to further study on glutathione.

关键词(KeyWords)： 谷胱甘肽;生理功能;应用;制备;检测
glutathione;physiological functions;application;preparation;detection

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 贾贞,王丹,游松
JIA Zhen,WANG Dan,YOU Song(School of Life Science and Biopharmaceutics

参考文献(References)：

• [1]冮洁,单立峰.谷胱甘肽的制备及其应用[J].饲料工业,2007,28(15):15-17.
• [2]沈亚领,李爽,迟莉丽.谷胱甘肽的应用与生产[J].工业微生物,2000,30(2):42-45.
• [3]吴坚平,林建平,岑沛霖.谷胱甘肽的生物合成[J].化工进展,1999,(1):38-41.
• [4]LI W,BOLLECKER S S,SCHOFIELD J D.Glutathioneand related thiol compounds.I.Glutathione and relatedthiol compounds in flour[J].Journal of Cereal Science,2004,(39):205-212.
• [5]汤亚杰,徐小玲,李冬生.发酵法生产谷胱甘肽的研究进展[J].食品与发酵工业,2007,33(1):75-79.
• [6]袁尔东,郑建仙.功能性食品基料-谷胱甘肽的研究进展[J].食品与发酵工业,1999,25(5):52-57.
• [7]王大慧,卫功元.谷胱甘肽的应用前景及生产研究现状[J].化学与生物工程,2004,3:10-12.
• [8]CAMERA E,PICARDO M.Analytical methods to in-vestigate glutathione and related compounds in biologi-cal and pathological processes[J].Journal of Chroma-tography B,2002,781:181-206.
• [9]RAGHUNATHAN V K,ELLIS E M,JUSTICE N A T,et al.Involvement of reduced glutathione and glutathi-one reductase in the chronic toxicity of hexavalent chro-mium to monocytes in vitro[J].Toxicology,2007,23(2-3):105-106.
• [10]REGAN R F,GUO Y P.Potentiation of excitotoxicinjury by high concentrations of extracellular reducedglutathione[J].Neuroscience,1999,91(2):463-470.
• [11]钱屹崟,徐新才,邵利坚,等.还原型谷胱甘肽减轻化疗毒副反应的疗效观察[J].现代肿瘤医学,2008,16(2):286-288.
• [12]严宏.谷胱甘肽与白内障[J].中华实用眼科杂志,1998,16(10):578-580.
• [13]胡欣.还原型谷胱甘肽与糖尿病[J].医学综述,2006,l2(l6):995-996.
• [14]MATTIA G D,BRAVI M C,LAURENTI O,et al.Influence of reduced glutathione infusion on glucosemetabolism in patients with non-insulin-dependentdiabetes mellitus[J].Metabolism,1998,147(8):993-997.
• [15]PONSODAX,BORTR,JOVER R,et al.Increased tox-icity of cocaine on human hepatocytes induced by etha-nol:role of GSH[J].Biochemical Pharmacology,1999,58(10):1 579-1 585.
• [16]SPRIETSMA J E.Cysteine,glutathione(GSH)and zincand copper ions together are effective,natural,intracel-lular inhibitors of(AIDS)viruses[J].Medical Hypothe-ses,1999,52(6):529-538.
• [17]FRATERNALE A,PAOLETTI M F,CASABIANCA A,et al.Inhibition of murine AIDS by pro-glutathione(GSH)molecules[J].Antiviral Research,2008,(77):120-127.
• [18]EVERY D,MORRISON S C,SIMMONS L D,et al.Distribution of glutathione in millstreams and relation-ships to chemical and baking properties of flour[J].Cereal Chem,2006,83(1):57-61.
• [19]周宇光,付国平,肖仔君.谷胱甘肽的生产与应用研究进展[J].食品工业科技,2003,24(3):89-91.
• [20]刘国琴,陈洁,赵雷,等.小麦胚芽中谷胱甘肽提取方法探讨及工艺条件优化[J].河南工业大学学报,2007,28(2):1-5.
• [21]安贤惠.还原型谷胱甘肽提取方法初探[J].淮海工学院学报,2003,12(2):49-52.
• [22]周惠明,朱科学.小麦胚中谷胱甘肽的纯化与富集的研究[J].中国粮油学报,2003,18(1):17-20.
• [23]邱雁临,殷伟,潘飞,等.吸附层析法从啤酒废酵母中提取谷胱甘肽[J].生物技术,2005,15(1):49-51.
• [24]刘宜锋,曹新志.新型食品添加剂-谷胱甘肽[J].福州大学学报,2002,30:714-721.
• [25]肖强,杨群伟,肖敏君.一种生物工程法生产谷胱甘肽的方法:中国,200410079363.5[P].2006-04-19.
• [26]吴梧桐,劳兴珍,郑珩,等.用酶工程技术生物合成谷胱甘肽的方法;中国,200710020902.1[P].2007-10-17.
• [27]陈坚,卫功元,李寅,等.微生物发酵法生产谷胱甘肽[J].无锡轻工大学学报,2004,23(5):104-109.
• [28]陈雪,赵文杰,冯军,等.谷胱甘肽产生菌的菌种选育[J].中国医药工业杂志,2007,38(7):481-483.
• [29]陈坚,李寅,卫功元,等.一种提高产朊假丝酵母发酵生产谷胱甘肽产量的方法:中国,03113418.1[P].2003-10-22.
• [30]林建平,游建锋,盛晓霞,等.利用啤酒废酵母间歇发酵合成谷胱甘肽的研究[J].高校化学工程学报,2003,17(6):689-694.
• [31]曹新志,陈彦.荧光法测定小麦胚中的谷胱甘肽[J].粮食与饲料工业,2001,l1:46-47.
• [32]范崇东,王淼,卫功元,等.谷胱甘肽测定方法研究进展[J].生物技术,2004,14(1):68-70.
• [33]陈俭梅,周琳.几种谷胱甘肽的检测方法[J].山东食品发酵,2007,146:26-29.
• [34]刘娟,王雅琴,刘刚,等.发酵液中还原型谷胱甘肽三种测定方法的改进及其比较[J].北京化工大学学报,2004,31(3):35-38.
• [35]黄颖,段建平,杨明灿,等.毛细管电泳法测定黄瓜和西红柿中的谷胱甘肽[J].色谱,2003,21(5):510-512.
• [36]王春,严伟民.注射用还原型谷胱甘肽质量标准分析方法-HPLC法的建立与验证[J].中国临床药学杂志,2005,14(3):167-169.
• [37]林志兴,吕永琴,谭天伟.高效液相色谱-蒸发光散射检测器定量分析谷胱甘肽含量[J].食品与发酵,2007,33(4):119-121.