袁霞,刘唯芬,张艳君
YUAN Xia,LIU Wei-fen,ZHANG Yan-jun(School of Vocational and Technology

• 1:沈阳药科大学高等职业技术学院

摘要(Abstract)：

目的建立高效液相色谱法测定刺五加中紫丁香苷和异秦皮啶的含量。方法采用KromasilC18色谱柱(250 mm×4.6 mm,5μm);流动相为乙腈(A)-体积分数为1%乙酸水溶液(B),梯度洗脱;流速为1.0 mL.min-1;检测波长为265 nm(0 min),341nm(11 min)。结果紫丁香苷和异秦皮啶质量浓度分别在26.0～435 mg.L-1(r=0.999 7)和0.270～4.50 mg.L-1(r=0.999 6)内与峰面积呈良好的线性关系;平均回收率分别为98.01%(RSD=1.2%,n=6)和97.53%(RSD=1.1%,n=6)。结论该方法可同时测定刺五加药材中紫丁香苷和异秦皮啶的含量。
Objective To develop an HPLC method for determination of syringin and isofraxidin in Acanthopanax senticosus by RP-HPLC.Methods The chromatographic separation was carried out on a Kromasil C18 column(250 mm×4.6 mm,5 μm).The mobile phase consisted of acetonitrile and 1% acetic acid with gradient elution was used;the flow rate was 1.0 mL · min-1.The UV detector was set at 265 nm(0 min)and 341 nm(11 min).Results A good linear relationship of syringin was in the range of 26.0-435 mg · L-1(r=0.999 7)and that of isofraxidin was in the range of 0.270-4.50 mg · L-1(r=0.999 6).The average recoveries of syringin and isofraxidin were 98.01%(RSD=1.2%,n=6)and 97.53%(RSD=1.1%,n=6),respectively.Conclusions The method is suitable for the simultaneous determination of syringin and isofraxidin in Acanthopanax senticosus.

关键词(KeyWords)： 高效液相色谱法;刺五加;紫丁香苷;异秦皮啶;含量测定
HPLC;Acanthopanax senticosus;syringin;isofraxidin;content determination

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 袁霞,刘唯芬,张艳君
YUAN Xia,LIU Wei-fen,ZHANG Yan-jun(School of Vocational and Technology

DOI: 10.14066/j.cnki.cn21-1349/r.2012.01.007

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