张雅昕,王龙,刘克非,王范,徐晖,邓意辉
ZHANG Ya-xin,WANG Long,LIU Ke-fei,WANG Fan,XU Hui,DENG Yi-hui(School of Pharmacy

• 1:沈阳药科大学药学院

摘要(Abstract)：

目的建立人参皂苷Re蛋清蛋白纳米混悬剂中药物含量的测定方法。方法采用DiamonsilC18色谱柱(250 mm×4.6 mm,5μm),流动相为乙腈-体积分数为0.05%磷酸水溶液(体积比25∶75),流速1.0 mL.min-1,紫外检测波长203 nm,柱温25℃。结果在本实验条件下,制剂中辅料对药物含量的测定无干扰,人参皂苷Re质量浓度在44.40~177.6 mg.L-1内线性关系良好(r=0.999 9,n=5),药物平均加样回收率为100.1%,RSD为0.84%(n=9)。结论此方法准确可靠、简便易行、重现性好,可用于人参皂苷Re蛋清蛋白纳米混悬剂中药物含量的测定。
Objective To establish an HPLC method for the content determination of ginsenoside Re in egg albumen nanosuspensions.Methods The separation was performed with a Diamonsil C18 column(250 mm×4.6 mm,5 μm) at 25 ℃,and the mobile phase was acetonitrile-0.05% phosphoric acid(V∶V=25∶75),the flow rate was 1.0 mL·min-1 and detective wavelength was set at 203 nm.Results The linear range of ginsenoside Re was 44.40-177.6 mg·L-1(r=0.999 9,n=5).The average recovery of ginsenoside Re was 100.1%,and RSD was 0.84%(n=9).Conclusions The method is simple,accurate and reproducible,and it can be used for determination of ginsenoside Re in egg albumen nanosuspensions.

关键词(KeyWords)： 人参皂苷Re;蛋清蛋白纳米混悬剂;高效液相色谱法;含量测定
ginsenoside Re;egg albumen nanosuspension;HPLC;content determination

Abstract:

Keywords:

基金项目(Foundation): 国家重点基础研究发展计划(973计划)资助项目(2009CB930300)

作者(Author): 张雅昕,王龙,刘克非,王范,徐晖,邓意辉
ZHANG Ya-xin,WANG Long,LIU Ke-fei,WANG Fan,XU Hui,DENG Yi-hui(School of Pharmacy

DOI: 10.14066/j.cnki.cn21-1349/r.2010.10.008

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