长白红景天内生真菌Fusariums sp.HJT-P-5次级代谢产物研究Studies on the secondary metabolites of endophytic fungus Fusariums sp. HJT-P-5
魏磊,刘硕,靳元春,冯宝民,卢轩
WEI Lei,LIU Shuo,JIN Yuanchun,FENG Baomin,LU Xuan
摘要(Abstract)：
目的研究长白红景天植物内生真菌Fusarium sp. HJT-P-5次级代谢产物,以期得到结构新颖的先导化合物。方法采用硅胶柱色谱、ODS反相柱色谱和高效液相色谱等方法进行分离纯化,通过理化性质和波谱数据分析鉴定化合物的结构。结果内生真菌Fusarium sp. HJT-P-5次级代谢产物中分离得到7个化合物,分别鉴定为:Fusarnaphthoquinone A(Ⅰ),5-ydroxydihydrofusarubin A(Ⅱ),5-hydroxydihydrofusarubin C(Ⅲ),4a,10a-dihydrofusarubine(Ⅳ),3-acetoxy-4-deoxybostrycin(Ⅴ),Javanicin(Ⅵ),Fusarnaphthoquinone B(Ⅶ)。结论化合物Ⅰ～Ⅶ均为首次从红景天内生微生物中分离得到。
Objective To study the secondary metabolites from the fungi Fusarium sp. HJT-P-5 in the plant Rhodiola angusta Nakai,in order to obtain lead compounds. Methods The compounds were isolated by silica gel,ODS reverse phase column chromatography and high performance liquid chromatography( HPLC). The structures of the compounds were identified by physic-chemical properties and spectroscopic data. Results Seven compounds were isolated from Fusarium sp. HJT-P-5 secondary metabolites,which were identified as fusarnaphthoquinone A( Ⅰ),5-hydroxydihydrofusarubin A( Ⅱ),5-hydroxydihydrofusarubin C( Ⅲ),4 a,10 a-dihydrofusarubine( Ⅳ),3-acetoxy-4-deoxybostrycin( Ⅴ),javanicin( Ⅵ),and fusarnaphthoquinoneB( Ⅶ). Conclusion Compounds Ⅰ-Ⅶ were isolated from the Rhodiola endophytic fungi for the first time.
关键词(KeyWords)：
植物内生真菌;镰刀霉属;次级代谢产物
endophytic fungi;Fusarium sp.;Secondary metabolites
基金项目(Foundation): 国家自然科学基金资助项目(31540006,31270398);; 辽宁省教育厅科研资助项目(L2015024)
作者(Author):
魏磊,刘硕,靳元春,冯宝民,卢轩
WEI Lei,LIU Shuo,JIN Yuanchun,FENG Baomin,LU Xuan
DOI: 10.14066/j.cnki.cn21-1349/r.2018.10.005
参考文献(References)：
- [1]杨镇,曹君.植物内生菌及其次级代谢产物的研究进展[J].微生物学杂志,2016,36(4):1-6.
- [2] DEMAIN A L. In biodiversity:new leads for pharmaceutical and agrochemical industries[M]. United Kingdom:The Royal Society of Chemistry,2000:3-16.
- [3] NEWMAN D J,CRAGG G M. Natural products as sources of new drugs over the last 25 years[J]. Journal of Natural Products,2007,70(3):461-477.
- [4] TRISUWAN K, KHAMTHONG N, RUKACHAISIRIKUL V,et al. Anthraquinone,cyclopentanone,and naphthoquinone derivatives from the sea fan-derived fungi Fusarium sp. PSU-F14 and PSU-F135[J].Journal of Natural Products,2010,73(9):1507-1511.
- [5] KORNSAKULKARN J, DOLSOPHON K,BOONYUEN N,et al. Dihydronaphthalenones from endophytic fungus Fusarium sp. BCC14842[J]. Tetrahedron,2011,67(39):7540-7547.
- [6] TATUM J H,BAKER R A. Naphthoquinones produced by Fusarium solani isolated from citrus[J]. Phytochemistry,1983,22(2):543-547.
- [7] YANG K L,WEI M Y,SHAO C L,et al. Antibacterial anthraquinone derivatives from a sea anemone-derived fungus Nigrospora sp.[J]. Journal of Natural Products,2012,75(5):935-941.
- [8] HASHIMOTO J,MOTOHASHI K,SAKAMOTO K,et al. Screening and evaluation of new inhibitors of hepatic glucose production[J]. Journal of Antibiotics,2009,62(11):625-629.