靖博宇,张颖,王光函,赵玥,杨瑞,邹桂欣,鞠俭奎,李国信
JING Boyu,ZHANG Ying,WANG Guanghan,ZHAO Yue,YANG Rui,ZOU Guixin,JU Jiankui,LI Guoxin

• 1:辽宁省中医药研究院

摘要(Abstract)：

目的 通过对抗感颗粒中牛蒡子、黄芩、连翘、甘草等药材进行薄层色谱(TLC)鉴别、对有效成分黄芪苷和牛蒡苷进行含量测定的研究，建立科学合理的抗感颗粒有效组分质量控制方法。方法 采用薄层色谱法鉴别牛蒡子、连翘和甘草药材；采用高效液相色谱法对方中牛蒡苷和黄芩苷进行含量测定。结果 有效成分黄芩苷和牛蒡苷分别在88.76～887.6、33.68～336.8 mg·L~(-1)范围内线性关系良好(r=0.999 9),平均加样回收率分别为100.1%、100.3%,RSD分别为1.3%、1.5%(n=6)。薄层鉴别图谱斑点圆整且分离度好，阴性对照无干扰。结论 该方法准确可靠，重复性好，专属性强，可用于抗感颗粒的质量控制。
Objective To establish a scientific and reasonable quality control method of Kanggan Granules(Arctii Fructus, Scutellaria Radix, Forsythiae Fructus, Glycyrrhiza, etc) through the qualitative and quantitative research of thin layer identification(TLC) and content determination of Kanggan Granules.Methods The qualitative identification of Arctii Fructus, Forsythiae Fructus, Glycyrrhiza were identified by TLC method.The simultaneous content determination of baicalin and arctiin in Kanggan granules were performed by HPLC.Results The linear range of baicalin and arctiin were 88.76-887.6、33.68-336.8 mg·L~(-1)(r=0.999 9),respectively.The average recoveries were 100.1%,100.3% with the RSD of 1.3%,1.5%(n=6),respectively.The TLC spots were clear without negative interference.Conclusion The simple, accurate, reproducible and stable method can be used for the quality control of Kanggan Granules.

关键词(KeyWords)： 抗感颗粒;牛蒡苷;黄芩苷;连翘苷;甘草次酸;TLC;HPLC;含量测定
Kanggan Granules;arctiin;baicalin;forsythin;glycyrrhetinicacid;TLC;HPLC;content determination

Abstract:

Keywords:

基金项目(Foundation): 辽宁省重点研发计划项目(2020JH2/10300090)

作者(Author): 靖博宇,张颖,王光函,赵玥,杨瑞,邹桂欣,鞠俭奎,李国信
JING Boyu,ZHANG Ying,WANG Guanghan,ZHAO Yue,YANG Rui,ZOU Guixin,JU Jiankui,LI Guoxin

DOI: 10.14066/j.cnki.cn21-1349/r.2021.0453

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