反相离子对色谱法测定青木香中马兜铃酸A的含量Determination of aristolochic acid A in Radix Aristolochiae by RP HPLC
赵霓,来剑峰,毕开顺
ZHAO Ni; LAI Jian feng; BI Kai shun (Department of Pharmacy;
摘要(Abstract)：
建立反相离子对色谱法测定青木香中马兜铃酸A含量。采用HypersilC18色谱柱(2 0 0mm× 4 6mm ,5 μm) ,以甲醇∶1 2 8%四丁基溴化氨∶pH 3 6醋酸 醋酸钠缓冲液 (体积比2∶1∶1)为流动相 ,流速 1 0mL/min ,检测波长 316nm。线性范围 2 0 8～ 10 4 0 μg/mL(r =0 9998) ,样品平均加样回收率为 97 0 % (n =6 ) ,精密度RSD为 1 5 % (n =6 )。该法简便、快速、准确 ,适用于青木香中马兜铃酸A的定量分析
The paper is to establish a method using RP HPLC for determination of the content of aristolochic acid A in Radix Aristolochiae. The instrument used was Waters Model 510 HPLC with a Hypersil C 18 column( 200 mm × 4 6 mm , 5 μm ).The mobile phase was methanol:1 28% tetrabutylammonium bromide:acetic acid sodium acetate buffer solution (pH 3 6) (volumn radio 2∶1∶1) and the flow rate was 1 0 mL/min .The UV detection wavelenth was 316 nm and the column temperature was room temperature. The calibration curve was linear( r= 0 999 8 ) within the range of 20 8～ 104 0 μg/mL for aristolochic acid A.The mean recovery was 97 0%( n= 6) and the precision (RSD) was 2 4%( n= 6). The method was convenient,quick,accurate and suitble for the quality control of the aristolochic acid A in Radix Aristolochiae.
关键词(KeyWords)：
青木香;马兜铃酸A;反相离子对色谱法
Radix Aristolochiae; aristolochic acid A; RP H?
基金项目(Foundation):
作者(Authors):
赵霓,来剑峰,毕开顺
ZHAO Ni; LAI Jian feng; BI Kai shun (Department of Pharmacy;
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