陈秋,王涛,葛丹丹,贺伟,吴春华,张祎
CHEN Qiu,WANG Tao,GE Dan-dan,HE Wei,WU Chun-hua,ZHANG Yi(Key Laboratory of Traditional Chinese Medicinal Chemistry and Analytical Chemistry of Tianjin

• 1:天津市中药化学与分析重点实验室

摘要(Abstract)：

目的对火绒草的化学成分进行研究,为进一步开发利用该植物资源提供依据。方法采用反复正相硅胶、反相ODS、Sephadex LH-20等柱色谱以及高效液相色谱法等手段进行分离纯化,并通过理化性质与光谱分析鉴定化合物的结构。结果从火绒草体积分数为70%的乙醇溶液提取物中又分离鉴定了7个黄酮类化合物,分别为芹菜素(apigenin,1)、山柰酚-3-O-(6″-O-乙酰基)-β-D-吡喃葡萄糖苷[kaempferol-3-O-(6″-O-acetyl)-β-D-glucopyranoside,2]、山柰酚-3-O-(6″-O-反式对香豆酰基)-β-D-吡喃葡萄糖苷[kaempferol-3-O-(6″-O-trans-p-coumaroyl)-β-D-glucopyranoside,3]、山柰酚-3-O-(6″-O-顺式对香豆酰基)-β-D-吡喃葡萄糖苷[kaempferol-3-O-(6″-O-cis-p-coumaroyl)-β-D-glucopyranoside,4]、槲皮素-7-O-β-D-吡喃葡萄糖苷(quercetin-7-O-β-D-glucopyranoside,5)、槲皮素-3-甲氧基-7-O-β-D-吡喃葡萄糖苷(quercetin-3-me-thoxy-7-O-β-D-glucopyranoside,6)、槲皮万寿菊素-7-O-β-D-吡喃葡萄糖苷(quercetagetin-7-O-β-D-glucopyranoside,7)。结论化合物1为首次从火绒草植物中分离得到,化合物2～7为首次从火绒草属植物中分离得到。
Objective To study the chemical constituents from Leontopodium leontopodioides(Willd.)Beauv.Methods The compounds were isolated by chromatographic methods such as silica gel,ODS,Sephadex LH-20 and HPLC,and identified by the chemical and physical methods,especially spectral analysis.Results Seven flavonoids were isolated.The structures of them were identified as apigenin(1),kaempferol-3-O-(6″-O-acetyl)-β-D-glucopyranoside(2),kaempferol-3-O-(6″-O-trans-p-coumaroyl)-β-D-glucopyranoside(3),kaempferol-3-O-(6″-O-cis-p-coumaroyl)-β-D-glucopyranoside(4),quercetin-7-O-β-D-glucoside(5),quercetin-3-methyloxy-7-O-β-D-glucopyranoside(6),and quercetagetin-7-O-β-D-glucopyranoside(7),respectively.Conclusions Compound 1 is obtained from the plant for the first time,and compounds 2-7 are obtained from Leontopodium genus for the first time.

关键词(KeyWords)： 火绒草;化学成分;黄酮;结构鉴定
Leontopodium leontopodioides(Willd.)Beauv.;chemical constituent;flavonoid;structure identification

Abstract:

Keywords:

基金项目(Foundation): 天津市自然科学基金资助项目(09JCYBJC16300);; 天津市高等学校科技发展基金计划资助项目(20090201)

作者(Author): 陈秋,王涛,葛丹丹,贺伟,吴春华,张祎
CHEN Qiu,WANG Tao,GE Dan-dan,HE Wei,WU Chun-hua,ZHANG Yi(Key Laboratory of Traditional Chinese Medicinal Chemistry and Analytical Chemistry of Tianjin

DOI: 10.14066/j.cnki.cn21-1349/r.2012.02.010

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