离子色谱法同时测定牛乳及牛乳制品中硫氰酸盐和高氯酸盐Simultaneous determination of thiocyanide and perchlorate in the milk by ion chromatography
丛鑫,苏葳艺,赵晓云,李欣南,赵怀清
CONG Xin1,SU Wei-yi2,ZHAO Xiao-yun3,LI Xin-nan2,ZHAO Huai-qing1*(1.School of Pharmacy
摘要(Abstract)：
目的建立牛乳及牛乳制品中硫氰酸盐和高氯酸盐的测定方法。方法采用离子色谱法,Ion-Pac ASl6(250 mm×4 mm)分析柱,氢氧化钾溶液为淋洗液,梯度模式进行洗脱,电导检测器检测。结果牛乳中硫氰酸盐和高氯酸盐的检测限可达到0.050 mg.L-1和0.025 mg.L-1,且两者在0.5～50.0 mg.L-1内线性关系良好。结论该方法适用于牛乳及牛乳制品中硫氰酸盐和高氯酸盐的测定。
Objective To develope a method for determination of thiocyanide and perchlorate in the milk.Methods The milk was concentrated after extracted with acetonitrile and purified by solid-phase extraction and then determined on a hydrophobic anion-exchange column.The separation was performed with a KOH eluent in linear gradient.The detection was performed by a conductivity detection mode.Results The detection limits were 0.05 mg · L-1 for thiocyanide and 0.025 mg · L-1for perchlorate respectively.The calibration curves were linear from 0.5-50.0 mg · L-1 for thiocyanide and perchlorate.Conclusions This method is specific,sensitive,and suitable for determination of thiocyanide and perchlorate in the milk or its products.
关键词(KeyWords)：
硫氰酸盐;高氯酸盐;离子色谱
thiocyanide;perchlorate;ion chromatography
基金项目(Foundation):
作者(Author):
丛鑫,苏葳艺,赵晓云,李欣南,赵怀清
CONG Xin1,SU Wei-yi2,ZHAO Xiao-yun3,LI Xin-nan2,ZHAO Huai-qing1*(1.School of Pharmacy
DOI: 10.14066/j.cnki.cn21-1349/r.2013.08.001
参考文献(References)：
- [1]姜苏,李院生,马红梅,等.环境中高氯酸盐的来源,污染现状及其分析方法[J].地球科学进展,2010,25(6):617-624.
- [2]蔡亚岐,史亚利,张萍,等.高氯酸盐的环境污染问题[J].化学进展,2006,18(11):1554-1564.
- [3]SANCHEZ C A,BLOUNT B C,VALENTIN-BLA-SIN L,et al.Perchlorate in the feed-dairy continuumof the southwestern United States[J].Journal of Agri-cultural and Food Chemistry,2008,56:5443-5450.
- [4]帕提曼.卡吾力,古丽曼.木哈买提拜,贾新建.离子色谱法测定牛奶中硫氰酸根离子[J].饲料工业,2011,19:43-44.
- [5]KIRK A B.Environment perchlorate:why it matters[J].Anal Chim Acta,2006,567:4-12.
- [6]KIRK A B,MARTINELANGO P K,TIAN K,et al.Perchlorate and iodide in dairy and breast milk[J].Environmental Science and Technology,2005,39(7):2011-2017.
- [7]KIRK A B,SMITH E E,TIAN T A,et al.Perchloratein milk[J].Environmental Science and Technology,2003,37(21):4979-4981.
- [8]SHI Ya-li,ZHANG Ping,WANG Ya-wei,et al.Per-chlorate in sewage sludge,rice,bottled water and milkcollected from different areas in China[J].Environ-ment International,2007,33:955-962.
- [9]黄玉贤,白虹,孟淑珍,等.乳中硫氰酸盐测定方法的研究报告[J].黑龙江畜牧科技,1998,3:8.
- [10]张萍,史亚利,蔡亚岐,等.离子色谱-质谱联用测定牛奶中的高氯酸根、溴酸根和碘离子[J].分析测试学报,2007,5:690-693.
- [11]许牡丹,毛跟年.食品安全行与分析检测[M].北京:化学工业出版社,2005:40-41.