HPLC法同时测定红茶中6种活性成分的含量Determination of the content of six active components in black tea by HPLC
吴越,陈晓辉,钱忠直,宋曼铜,毕开顺
WU Yue1,CHEN Xiao-hui1,QIAN Zhong-zhi2,SONG Man-tong1,BI Kai-shun1(1.School of Pharmacy
摘要(Abstract)：
目的建立同时测定红茶中6种活性成分含量的方法。方法采用HPLC法。色谱柱为Kro-masil C18柱(250 mm×4.6 mm,5μm);流动相为甲醇-体积分数0.05%磷酸水溶液,梯度洗脱;流速1.0 mL.min-1;检测波长278 nm;柱温40℃。结果在此色谱条件下,各组分在60 min内均得到较好分离。没食子酸、可可碱、咖啡因、表没食子儿茶素没食子酸酯、表儿茶素、表儿茶素没食子酸酯质量浓度分别在3.1～78.2 mg.L-1、4.7～116.6 mg.L-1、55.3～1 382.5 mg.L-1、4.1～101.9 mg.L-1、19.3～482.0 mg.L-1、13.5～338.5 mg.L-1内与峰面积呈良好的线性关系,平均回收率分别为96.5%、97.65%、99.9%、96.6%、96.2%、98.3%(n=9),RSD均小于2.0%。结论本方法简单、准确、重复性好,可用于红茶的质量控制。
Objective To develop an HPLC method for the quantification of six active components in black tea.Methods The determination was performed on a Kromasil C18 column(250 mm×4.6 mm,5 μm).Methanol and 0.05% phosphoric acid were used as the mobile phases with a gradient elution.The flow rate was 1.0 mL·min-1.The UV detection wavelength was at 278 nm.The column oven temperature was at 40 ℃.Results Six components were separated commendably in 60 min.The calibration curves of gallic acid,theobromine,caffeine,epigallocatechin gallate,epicatechin and epicatechin gallate were in good linearity over the range of 3.1-78.2,4.7-116.6,55.3-1382.5,4.1-101.9,19.3-482.0,13.5-338.5 mg·L-1,respectively.The average recoveries were 96.5%,97.65%,99.9%,96.6%,96.2% and 98.3%,respectively,and the RSD were less than 2.0%.Conclusions The method is simple,accurate and reproducible,and can be used to control the quality of black tea.
关键词(KeyWords)：
红茶;茶多酚;生物碱;高效液相色谱法;含量测定
black tea;tea polyphenol;alkaloid;HPLC;content determination
基金项目(Foundation):
作者(Author):
吴越,陈晓辉,钱忠直,宋曼铜,毕开顺
WU Yue1,CHEN Xiao-hui1,QIAN Zhong-zhi2,SONG Man-tong1,BI Kai-shun1(1.School of Pharmacy
DOI: 10.14066/j.cnki.cn21-1349/r.2010.09.011
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