郝桂明,李欢欣,赵春杰,邹宇清
HAO Gui ming 1, LI Huan xin 1, ZHAO Chun jie 1, ZOU Yu qing 2 (1.School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China; 2.Dalian Meiluo New and Specific Drug Co., Ltd., Dalian 116011, China)

• 1:沈阳药科大学药学院
• 2:沈阳药科大学药学院
• 3:沈阳药科大学药学院
• 4:大连美罗股份新药特药公司 辽宁沈阳110016

摘要(Abstract)：

目的建立一种用反相高效液相色谱法测定党参中苍术内酯Ⅲ含量的方法。方法采用Kro masilC18(2 5 0mm× 4 6mm ,5 μm)色谱柱 ,甲醇 水 (60∶40 ,V∶V)为流动相 ,以尼莫地平为内标 ,检测波长 2 2 0nm。结果线性范围为 0 5～ 1 0 0mg/L ,三水平的平均回收率 (% )分别为 82 1、85 4和88 4,RSD(% )分别为 2 9、1 3和 2 7。结论本法简便 ,准确 ,可作为党参药材的质量控制方法。
Objective To develop a reversed phase high performance liquid chromatographic method for the determination of the content of atractylenoide Ⅲ,which was known as the active constituent of Codonopsis pilosula . Methods The mobile phase consisted of methanol and water (60∶40, V∶V ) and the nimodipine was used as the internal standard. The detector was operated at 220 nm. Results The calibration curves were linear in the range of 0 5～10 0 mg/L. The recoveries (%) were 82 1,85 4 and 88 4. The RSD were 2 88,1 31 and 2 67, respectively. Conclusions The method has good accuracy and repeatability and it can be used for the quality control of Codonopsis pilosula .

关键词(KeyWords)： 反相高效液相色谱法;党参;苍术内酯Ⅲ
RP HPLC; Codonopsis pilosula ;atractylenoide Ⅲ

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目 (3 9970 91 0 )

作者(Authors): 郝桂明,李欢欣,赵春杰,邹宇清
HAO Gui ming 1, LI Huan xin 1, ZHAO Chun jie 1, ZOU Yu qing 2 (1.School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China; 2.Dalian Meiluo New and Specific Drug Co., Ltd., Dalian 116011, China)

参考文献(References)：

• [1]中华人民共和国国家药典委员会.中华人民共和国药典:一部[Z].北京:化学工业出版社,2000.233.
• [2]王峥涛,徐国钧,难波恒雄,等.党参中苍术内酯Ⅲ的HPLC分析[J].中国药科大学学报,1991,23(1):48-50.
• [3]朱恩圆,贺庆,王峥涛,等.党参化学成分研究[J].中国药科大学学报,2001,32(2):94-96.