高广慧;王晓黎;董宇;慕善学;廉瑛;
GAO Guang-hui1,WANG Xiao-li1,DONG Yu1,MU Shan-xue2,LIAN Ying3(1.Liaoning Provincial Institute for Food And Drug Control,Shenyang 110023,China;2.Shenyang Pharmaceutical University,Shenyang 110026,China;3.Shenyang Yilian Xin Hai Pharmaceutical Co.,Ltd,Shenyang 110016,China)

• 1:辽宁省食品药品检验所
• 2:沈阳药科大学高职技术学院
• 3:沈阳医联药业公司

摘要(Abstract)：

目的建立毛细管气相色谱法测定羟乙基淀粉中2-氯乙醇的残留量。方法采用毛细管气相色谱法。色谱柱为DM-WAX弹性石英毛细管柱(30 m×0.25 mm,0.25μm);载气为氮气;检测器为ECD;进样口和检测器温度均为250℃;柱温为程序升温,初始温度90℃,保持12 min,以每分钟20℃升温至120℃,保持2 min。结果2-氯乙醇质量浓度在14.30¹19.2μg.L-1内线性关系良好,r=0.997 6;回收率为98.4%(n=9);检测限为9.54×10-3ng;定量限为2.86×10-2ng。结论本法灵敏度高,简单,易行,结果准确可靠,可为羟乙基淀粉的质量控制提供依据。
Objective To establish a GC method for the determination of 2-chloroethanol residue in hydroxyethyl starch.Methods A column of DM-WAX was used with ECD as detector;nitrogen was selected as carrier gas.The injector temperature and the detector temperature were 250 ℃;the programmed column temperature was set at 90 ℃ for 12 min and then to 120 ℃ at the rate of 20 ℃·min-1 followed by holding for 2 min.Results A good linearity was obtained at the concentration of 14.30-119.2 μg·L-1 with correlation of 0.997 6.The average recovery was 98.4%(n=9),the limit of detection was 9.54×10-3 ng;the limit of quantity was 2.86×10-2 ng.Conclusions This method is simple,accurate and feasible.It is very suitable for the quality control of 2-chloroethanol in hydroxyethyl starch.

关键词(KeyWords)： 气相色谱法;羟乙基淀粉;2-氯乙醇;溶剂残留
GC;hydroxyethyl starch;2-chloroethanol;residual solvent

Abstract:

Keywords:

基金项目(Foundation):

作者(Authors): 高广慧;王晓黎;董宇;慕善学;廉瑛;
GAO Guang-hui1,WANG Xiao-li1,DONG Yu1,MU Shan-xue2,LIAN Ying3(1.Liaoning Provincial Institute for Food And Drug Control,Shenyang 110023,China;2.Shenyang Pharmaceutical University,Shenyang 110026,China;3.Shenyang Yilian Xin Hai Pharmaceutical Co.,Ltd,Shenyang 110016,China)

DOI: 10.14066/j.cnki.cn21-1349/r.2010.05.017

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