孙彦君,周巍,陈虹,李占林,华会明
SUN Yan-jun1,ZHOU Wei1,CHEN Hong2,LI Zhan-lin1,HUA Hui-ming1(1.School of Traditional Chinese Materia Medica

• 1:沈阳药科大学中药学院
• 2:武警医学院生药教研室

摘要(Abstract)：

目的对桃儿七的95%(φ)乙醇提取物的化学成分进行研究。方法利用反复硅胶、SephadexLH-20、开放ODS柱色谱等方法进行分离纯化;根据理化性质及波谱分析对分离得到的化合物进行结构鉴定。结果分离得到8个黄酮类化合物,分别鉴定为山柰酚(kaempferol,1)、槲皮素(querce-tin,2)、杨梅素-3',4'-二甲醚(myricetin-3',4'-dimethyl ether,3)、3-O-甲基-槲皮素(3-O-methyl-quercetin,4)、山柰酚-3-O-β-D-葡萄糖苷(kaempferol-3-O-β-D-glucopyranoside,5)、槲皮素-3-O-β-D-葡萄糖苷(quercetin-3-O-β-D-glucopyranoside,6)、芦丁(rutin,7)、山柰酚-3-O-芸香糖苷(kaempfer-ol-3-O-rutinoside,8)。结论化合物3、4、8为首次从该属植物中分离得到。
Objective To isolate and identify the chemical constituents from the 95%(φ) EtOH extract of the roots and rhizomes of Sinopodophyllum emodi.Methods Compounds were isolated by various kinds of column chromatography on silica gel,Sephadex LH-20 and ODS,and their structures were elucidated by the physico-chemical characters and the spectral analysis.Results Eight flavonoids were obtained and identified as kaempferol(1),quercetin(2),myricetin-3′,4′-dimethyl ether(3),3-O-methyl-quercetin(4),kaempferol-3-O-β-D-glucopyranoside(5),quercetin-3-O-β-D-glucopyranoside(6),rutin(7),and kaempferol-3-O-rutinoside(8),respectively.Conclusions Compounds 3,4 and 8 are isolated from Sinopodophyllum genus for the first time.

关键词(KeyWords)： 桃儿七;化学成分;黄酮类;结构鉴定
Sinopodophyllum emodi;chemical constituent;flavonoid;structural identification

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(30873363)

作者(Author): 孙彦君,周巍,陈虹,李占林,华会明
SUN Yan-jun1,ZHOU Wei1,CHEN Hong2,LI Zhan-lin1,HUA Hui-ming1(1.School of Traditional Chinese Materia Medica

DOI: 10.14066/j.cnki.cn21-1349/r.2012.03.003

参考文献(References)：

• [1]应俊生.小檗科八角莲属和桃儿七属(新属)的研究[J].植物分类学报,1979,17(1):15-23.
• [2]李广民.介绍一种药用植物——桃儿七[J].植物学杂志,1975,2(2):30.
• [3]杨璐璐,泰兴卫,杨倩.桃儿七的研究现状及开发利用[J].解放军药学学报,2000,16(1):52-53.
• [4]ZHAO Chang-qi,CAO Wei,NAGATSU A,et al.Three new glycosides from Sinopodophyllum emodi(Wall.)Ying[J].Chem Pharm Bull,2001,49(11):1474-1476.
• [5]ZHAO Chang-qi,HUANG Jing,NAGATSU A,et al.Two new podophyllotoxin glucosides from Sinopodo-phyllum emodi(Wall.)Ying[J].Chem Pharm Bull,2001,49(6):773-775.
• [6]ZHAO Chang-qi,NAGATSU A,HATANO K,et al.New lignan glycosides from Chinese medicinal plant,Sinopodophyllum emodi[J].Chem Pharm Bull,2003,51(3):255-261.
• [7]SUN Yan-jun,LI Zhan-lin,CHEN Hong,et al.Threenew cytotoxic aryltetralin lignans from Sinopodophyl-lum emodi[J].Bioorg Med Chem Lett,2011,21(12):3794-3797.
• [8]ZHAO Chang-qi,CHAO Wei,HUANG Jing,et al.Flavonoids from Sinopodophyllum emodi(Wall.)Ying[J].Nat Med,2001,55(3):152.
• [9]易衍,巫鑫,王英,等.霸王花黄酮类成分研究[J].中药材,2011,34(5):712-716.
• [10]ZAHIR A,JOSSANG A,BODO B,et al.DNA topoi-somerase I inhibitors:cytotoxic flavones from Lethe-don tannaensis[J].J Nat Prod,1996,59(7):701-703.
• [11]AYANOGLU E,ULUBELEN A,CLARK W D,etal.Myricetin and quercetin methyl ethers from Haplo-pappus integerrimus var.punctatus[J].Phytochemis-try,1981,20(7):1715-1717.
• [12]ROITMAN J N,JAME L F.Chemistry of toxic rangeplants.Highly oxygenated flavonol methyl ethers fromG utierrezza mzcrocephala[J].Phytochemistry,1985,24(4):835-838.
• [13]李占林.文冠果果壳化学成分及其生物活性研究[D].沈阳:沈阳药科大学,2006:38-39.