梁统,覃燕梅,丁航,梁念慈,吴科峰,刘慧明
LIANG Tong1; QIN Yan-mei2; DING Hang2; LIANG Nian-ci2; WU Ke-feng1; LIU Hui-ming2 (1.Guangdong Laboratory for Research and Development of Natural Drugs;

• 1:广东医学院天然药物研究与开发实验室
• 2:广东医学院生化教研室
• 3:广东医学院生化教研室
• 4:广东医学院生化教研室

摘要(Abstract)：

目的研究侧柏总黄酮的抗炎作用。方法以二甲苯致小鼠耳肿胀及角叉菜胶诱发大鼠足爪肿胀模型分别探讨侧柏总黄酮对小鼠耳片肿胀及大鼠足爪肿胀的抑制作用。测定大鼠足爪组织中一氧化氮 (NO)及前列腺素E2 (PGE2 )的含量。结果侧柏总黄酮在 12 5～ 5 0 0mg·kg-1内呈剂量依赖地抑制二甲苯诱导的小鼠耳肿胀 ,2 5 0mg·kg-1剂量能显著抑制大鼠足爪肿胀 ,其作用强于2 0mg·kg-1的地塞米松。侧柏总黄酮能抑制大鼠炎症足爪组织NO及PGE2 的生物合成。结论侧柏总黄酮具有较强的抗炎作用 ,其抗炎作用可能与抑制NO及PGE2 的生物合成或释放有关。
Objective To investigate the anti-inflammatory effects of total flavonoids isolated from Platycladus orientalis.Methods The inflammatory models in mice and swelling of rats′ hind paws were induced by dimethylbenzene and by carrageenan respectively.The contents of PGE 2 and NO in the hind paws of rats were measured.Results The total flavonoids isolated from Platycladus orientalis remarkably inhibited the ear edema induced by dimethylbenzene in mice at the dose of 12.5～ 50.0 mg·kg -1 and the swelling of rats′ hind paws induced by carrageenan at the dose of 25.0, 50.0 mg·kg -1.In the inflammatory model of a rat's hind paws induced by carrageenan,total flavonoids can decrease the contents of PGE 2 and NO in the hind paws of rats.Conclusion Total flavonoids isolated from Platycladus orientalis have an anti-inflammatory effect which may be related to decreasing the contents of PGE 2 and NO.

关键词(KeyWords)： 侧柏;总黄酮;前列腺素E2;一氧化氮
Platycladus orientalis(L.)Framco; flavonoids; PGE 2; NO

Abstract:

Keywords:

基金项目(Foundation): 广东省卫生厅基金项目 (A2 0 0 2 4 74 );; 湛江市科技攻关项目 (EK0 12 5 )

作者(Authors): 梁统,覃燕梅,丁航,梁念慈,吴科峰,刘慧明
LIANG Tong1; QIN Yan-mei2; DING Hang2; LIANG Nian-ci2; WU Ke-feng1; LIU Hui-ming2 (1.Guangdong Laboratory for Research and Development of Natural Drugs;

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