张杨;卢轩;李博;于大永;冯宝民;
ZHANG Yang;LU Xuan;LI Bo;YU Dayong;FENG Baomin;College of Life Science and Technology,Dalian University;

• 1:大连大学生命科学与技术学院

摘要(Abstract)：

目的研究荨麻科艾麻属植物珠芽艾麻的化学成分,以期得到具有抑制N1神经氨酸酶活性的化合物。方法采用硅胶柱色谱、凝胶柱色谱、高效液相色谱等方法进行分离纯化,通过理化性质和波谱数据分析鉴定化合物的结构。结果从荨麻科艾麻属植物珠芽艾麻分离得到了13个黄酮类化合物,分别鉴定为:金合欢素-7-O-芸香糖苷(acaetin-7-O-rutinoside,1)、木樨草素-7-O-β-D-葡萄糖苷(luteolin-7-O-β-D-glucopyranoside,2)、芹菜素-7-O-β-D-吡喃葡萄糖苷(apigenin-7-O-β-D-glucopyranoside,3)、芹菜素(apigenin,4)、山奈酚-3-O-β-D-葡萄糖苷(kaempferol-3-O-β-D-glucopyranoside,5)、山奈酚-3,7-O-α-L-二鼠李糖苷(kaempferol-3-O-β-D-glucopyranoside,6)、金丝桃苷(hyperoside,7)、槲皮素-7-O-β-D-6″-乙酰葡萄糖苷(quercetin-7-O-β-D-6″-acetylglucopyranoside,8)、槲皮素-3-O-β-D-葡萄糖苷(quercetin-3-O-β-D-glucopyranoside,9)、大豆素(daidzein,10)、大豆苷(daidzin,11)、染料木苷(kaemferitrin,12)、红车轴草素-7-O-β-D-吡喃葡萄糖苷(pratensein-7-O-β-D-glucopyranoside,13)。对化合物1-13的抑制N1神经氨酸酶活性进行了测定,结果显示化合物5、6、9具有较强的活性。结论化合物10-13为异黄酮类化合物,该类化合物为首次从荨麻科中分离得到。化合物1-13均为首次从珠芽艾麻中分离得到。其中化合物5、6、9抑制N1神经氨酸酶活性较强。
Objective To study the chemical constituents of the Laportea bulbifera( Urticaceae) and obtain compounds having the activity of inhibiting N1 neuraminidase. Methods The compounds were isolated by silica gel column chromatography,Sephadex LH-20 column chromatography and HPLC techniques. And their structures were identified by the physicochemical properties and spectral analysis. Results Thirteen compounds were obtained from Laportea bulbifera( Urticaceae). They were identified as acaetin-7-Orutinoside( 1),luteolin-7-O-β-D-glucopyranoside( 2),apigenin-7-O-β-D-glucopyranoside( 3),apigenin( 4),kaempferol-3-O-β-D-glucopyranoside( 5),kaempferol-3,7-O-α-L-dirhamnoside( 6),hyperoside( 7),quercetin-7-O-β-D-6″-acetylglucopyranoside( 8),quercetin-3-O-β-D-glucopyranoside( 9),daidzein( 10),daidzin( 11),kaemferitrin( 12) and pratensein-7-O-β-D-glucopyranoside( 13). The inhibition of N1 neuraminidase activity of Compound 1-13 was measured,and it was revealed that Compounds 5,6,and 9 have strong activities. Conclusion Compounds 10-13 were isoflavones,which were reported for the first time in Urticaceae. All the compounds were reported for the first time in this species. Among them,compounds 5,6,and 9 showed better effects on the inhibition of N1 neuraminidase activity.

关键词(KeyWords)： 珠芽艾麻;黄酮;异黄酮;N1神经氨酸酶抑制活性
Laportea bulbifera;flavonoid;isoflavone;anti-N1 neuraminidase activity

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(31540006,31270398);; 辽宁省教育厅科研项目(L2015024)

作者(Authors): 张杨;卢轩;李博;于大永;冯宝民;
ZHANG Yang;LU Xuan;LI Bo;YU Dayong;FENG Baomin;College of Life Science and Technology,Dalian University;

DOI: 10.14066/j.cnki.cn21-1349/r.2018.11.004

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