王倩,张红梅,于治国
WANG Qian 1; ZHANG Hong mei 2; YU Zhi guo 1 (1. School of Pharmacy;

• 1:沈阳药科大学药学院
• 2:阜新第二制药厂
• 3:沈阳药科大学药学院 辽宁沈阳110016
• 4:辽宁阜新123000

摘要(Abstract)：

目的用高效液相色谱法测定盐酸雷洛昔芬的含量。方法采用ODS柱 ,流动相为乙腈 0 0 1mol/L十二烷基硫酸钠溶液 (体积比 5 5∶4 5 ,冰醋酸调节 pH值至 4 0 ) ,在波长2 86nm检测。结果测定了盐酸雷洛昔芬的含量 ,平均含量为 99 9% (RSD =0 4 6 % ,n =3)。样品进样量在0 5～ 2 5 μg内 ,与峰面积具有良好的线性关系 ,线性回归方程为 :A =-0 31 + 2 0 3 0 3·W ,r =0 9997。结论本测定方法灵敏、准确 ,重现性好 ,适用于盐酸雷洛昔芬及其制剂的常规分析。
Objective To develop a reversed phase high performance liquid chromatographic method to determine the content of raloxifene hydrochloride. Method The mobile phase consisted of acetonitrile 0 01 mol/L sodium dodecyl sulfate (55∶45,adjusted to pH4 0 using glacial acetic acid ) and the UV absorbance was monitored at 286 nm . Result The average content was 99 9% with RSD=0 46%. Conclusion This assay demonstrated that the method had adequate sensitivity and selectivity to measure the concentrations of raloxifene hydrochloride and could be used in the quality control of raloxifene hydrochloride.

关键词(KeyWords)： 盐酸雷洛昔芬;高效液相色谱法;含量测定
raloxifene hydrochloride; RP HPLC; determination

Abstract:

Keywords:

基金项目(Foundation):

作者(Authors): 王倩,张红梅,于治国
WANG Qian 1; ZHANG Hong mei 2; YU Zhi guo 1 (1. School of Pharmacy;

参考文献(References)：

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