黄肖霄,牛超,高品一,李玲芝,明萌,宋少江
HUANG Xiao-xiao,NIU Chao,GAO Pin-yi,LI Ling-zhi,MING Meng,SONG Shao-jiang (School of Traditional Chinese Materia Medica

• 1:沈阳药科大学中药学院

摘要(Abstract)：

目的对蔷薇科山楂属植物山楂(Crataegus pinnatifidaBge.)的叶子部分的化学成分进行研究。方法运用硅胶、Sephadex LH-20柱色谱?ODS柱色谱、制备HPLC等分离手段进行化学成分的分离纯化,根据理化性质及波谱数据鉴定其结构。结果从山楂叶体积分数为80%乙醇的提取物中分离得到7个化合物。分别鉴定为18,19-seco,2α,3β,-dihydroxy-19-oxo-urs-11,13(18)-dien-28-oic acid(1)、3,9-dihydroxy-megastigma-5-ene(2)、(3S,5R,6R,7E)-megatsigmane-7-ene-3-hydrox-y-5,6-epoxy-9-O-β-D-glucopyranoside(3)、(Z)-3-hexenyl-6-O-β-D-xylopyranosyl-(1″-6′)-β-D-gluco-pyranoside(β-primeveroside)(4)、苯甲酸(5)、对羟基苯丙酸(6)、反式对羟基桂皮酸(7)。结论化合物1-4为首次从该属植物中分离得到,5-7为首次从该种植物中分离得到。
Objective To study the chemical constituents of the leaves of Crataegus pinnatifida Bge.Methods Seven compounds were isolated by chromatography on silica gel column,Sephadex LH-20,ODS chromatography column,PHPLC and established on the basis of physico-chemical constants and spectroscopic analysis.Results Seven compounds were isolated from 80% ethanol-water extract and their structures were identified as 18,19-seco,2α,3β,-dihydroxy-19-oxo-urs-11,13(18)-dien-28-oic acid(1),3,9-dihydroxy-megastigma-5-ene(2),(3S,5R,6R,7E)-megatsigmane-7-ene-3-hydroxy-5,6-epoxy-9-O-β-D-glucopyranoside(3),(Z)-3-hexenyl-6-O-β-D-xylopyranosyl-(1″-6′)-β-D-glucopyranoside(β-primeveroside)(4),benzoic acid(5),p-hydroxy-phenylpropionic acid(6),trans-p-hydroxy cinamic acid(7).Conclusions Compounds 14 are isolated from this genus for the first time and compounds 57 are isolated from this plant for the first time.

关键词(KeyWords)： 山楂叶;化学成分;结构鉴定
Crataegus pinnatifida Bge.leaves;chemical constituent;structural identification

Abstract:

Keywords:

基金项目(Foundation): “基于靶点的药物设计与研究”教育部重点实验室项目

作者(Author): 黄肖霄,牛超,高品一,李玲芝,明萌,宋少江
HUANG Xiao-xiao,NIU Chao,GAO Pin-yi,LI Ling-zhi,MING Meng,SONG Shao-jiang (School of Traditional Chinese Materia Medica

DOI: 10.14066/j.cnki.cn21-1349/r.2010.08.003

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