温晓丽,石辛孺,海青梅,周冰捷,刘阳,郭兴杰
WEN Xiaoli,SHI Xinru,HAI Qingmei,ZHOU Bingjie,LIU Yang,GUO Xingjie

• 1:沈阳药科大学药学院

摘要(Abstract)：

目的建立手性HPLC法分离苄氟噻嗪、甲氯噻嗪、卡维地洛和戊乙奎醚4种碱性药物异构体。方法使用Chiralpak IC手性固定相法,通过考察流动相中酸碱添加剂种类、极性醇类改性剂种类、流动相醇烷比例、柱温和流速等因素对异构体分离度的影响,对分离条件进行优化。结果在优化的色谱条件下,4种药物异构体均达到了基线分离。结论以Chiralpak IC柱为手性固定相的HPLC法适于分离上述4种碱性药物的异构体。
Objective To establish high performance liquid chromatography methods for the chiral separation of four basic drugs including bendroflumethiazide,methyclothiazide,carvedilol and penehyclidine hydrochloride. Methods With the application of Chiralpak IC column,the factors affecting separation process such as acidic and basic addictives,the types and ratios of mobile phase,column temperature and flowrate were investigated. Results Under the optimal condition,four basic drugs are completely separated.Conclusion Chiralpak IC column can achieve complete separation of these four basic drugs.

关键词(KeyWords)： 高效液相色谱;异构体分离;碱性药物;手性固定相
high performance liquid chromatography;isomer separation;alkaline drug;chiral stationary phase

Abstract:

Keywords:

基金项目(Foundation): 沈阳药科大学“大学生创新创业训练计划”资助项目(201610163076)

作者(Author): 温晓丽,石辛孺,海青梅,周冰捷,刘阳,郭兴杰
WEN Xiaoli,SHI Xinru,HAI Qingmei,ZHOU Bingjie,LIU Yang,GUO Xingjie

DOI: 10.14066/j.cnki.cn21-1349/r.2018.07.009

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