山楂核化学成分的分离与鉴定Isolation and identification of chemical constituents from seeds of Crataegus pinnatifida Bge.
赵雷,李玲芝,彭缨,宋少江
ZHAO Lei,LI Ling-zhi,PENG Ying,SONG Shao-jiang(School of Traditional Chinese Materia Medica
摘要(Abstract)：
目的对山楂(Crataegus pinnatifida Bge.)核中的化学成分进行研究。方法运用硅胶﹑Seph-adex LH-20、ODS柱色谱,制备HPLC,重结晶等分离手段进行分离纯化。根据理化性质及波谱数据鉴定其结构。结果从山楂核体积分数为70%乙醇提取物中分离得到4个化合物,分别鉴定为threo-1-(4-hydroxy-3-methoxyphenyl)-2-[4-(3-hydroxypropyl)-2-methoxyphenoxy]-1,3-propanediol(1)、erythro-1-(4-hydroxy-3-methoxyphenyl)-2-[4-(3-hydroxypropyl)-2-methoxyphenoxy]-1,3-pro-panediol(2)、3-(4-hydroxy-3-methoxyphenyl)-3-methoxypropane-1,2-diol(3)、2-[4-(3-hydroxyprop-yl)-2-methoxyphenoxy]propane-1,3-diol(4)。结论化合物1～4为首次从山楂属植物中分离得到。
Objective To study the chemical constituents from the seeds of Crataegus pinnatifida Bge.Methods The compounds were isolated by chromatography of silica gel,Sephadex LH-20,ODS column,preparative C18 HPLC and recrystallization,their structures were identified on the basis of physico-chemical constant and spectral analysis.Results Four compounds were isolated from 70% ethanol extract from seeds of C.pinnatifida and identified as threo-1-(4-hydroxy-3-methoxyphenyl)-2-[4-(3-hydroxypropyl)-2-methoxyphenoxy]-1,3-propanediol(1),erythro-1-(4-hydroxy-3-methoxyphenyl)-2-[4-(3-hydroxypropyl)-2-methoxyphenoxy]-1,3-propanediol(2),3-(4-hydroxy-3-methoxyphenyl)-3-methoxypropane-1,2-diol(3),2-[4-(3-hydroxypropyl)-2-methoxyphenoxy]propane-1,3-diol(4).Conclusions Compounds 1-4 are firstly isolated from Crataegus genus.
关键词(KeyWords)：
山楂核;化学成分;成分分离;结构鉴定
Crataegus pinnatifida;chemical constituent;isolation;structure identification
基金项目(Foundation):
作者(Author):
赵雷,李玲芝,彭缨,宋少江
ZHAO Lei,LI Ling-zhi,PENG Ying,SONG Shao-jiang(School of Traditional Chinese Materia Medica
DOI: 10.14066/j.cnki.cn21-1349/r.2012.01.002
参考文献(References)：
- [1]凌一揆.中药学[M].上海:上海科技出版社,1984:129.
- [2]CHIN Y W,CHAI H B,KELLER W J,et al.Lignansand other constituents of the fruits of Euterpe oleracea(Acai)with antioxidant and cytoprotective activities[J].J Agric Food Chem,2008,56(17):7759-7764.
- [3]LIYA L,SEERAM N P.Maple syrup phytochemicalsinclude lignans,coumarins,a stilbene,and other previ-ously unreported antioxidant phenolic compounds[J].JAgric Food Chem,2010,58(22):11673-11679.
- [4]黄正胜,张卫东,林生,等.鸡肉参的化学成分研究[J].中国中药杂志,2009,34(13):1672-1675.
- [5]BALTENWECK-GUYOT R,TRENDEL J M,AL-BRECHT P,et al.Glycosides and phenylpropanoidglycerol in Vitis vinifera Cv.Gew urztraminer w ine ag-ric[J].J Agric Food Chem,2000,48(12):6178-6182.
- [6]KOUNO I,YANAGIDA Y,SHIMONO S,et al.Phe-nylpropanoids from the barks of Illicium difengpi[J].Chem Pharm Bull,1992,40(9):2462-2464.