阮婧华,孙毓庆
RUAN Jing hua; SUN Yu qing (School of Pharmacy;

• 1:沈阳药科大学药学院
• 2:沈阳药科大学药学院 辽宁沈阳110016
• 3:辽宁沈阳110016

摘要(Abstract)：

目的探讨毛细管电泳法研究天然冬虫夏草用不同溶剂提取的效果及测定其中核苷及其碱基含量的可行性。方法采用Unimicro毛细管电泳仪 ,石英毛细管柱 ( 6 0cm× 75 μmI D ) ,运行缓冲液分别为 36mmol/L硼砂 1 5mmol/L磷酸二氢钠 ( pH 8 9) ,分离温度 2 0℃ ,分离电压1 5kV ,测定波长 2 5 4nm ,虹吸进样 1 5s。结果基线分离冬虫夏草不同溶剂提取的样品。腺苷、尿苷、尿嘧啶和次黄嘌呤含量分别为 0 30 %、0 2 8%、0 1 4 %、0 0 5 % ;平均回收率分别为 96 4 %、98 2 %、94 3%、95 1 % ;RSD分别为 0 89%、1 2 %、1 4 %、1 8% (n =6 )。结论该法准确 ,快速 ,灵敏度高 ,对名贵药冬虫夏草的质量控制有较重要意义。
Objective To determine nucleoside and their bases extracted with different solvents in Cordyceps sinensis by capillary electrophoresis (CE). Method Unimicro capillary electrophoresis appartus and fused silica capillary ( 60 cm × 75 μm I.D., 52 cm effective length) were used. The running buffer was 36 mmol/L borate 15 mmol/L sodium dihydrogen phosphate (pH 8 90). Fifteen kV was applied for the separation.The siphonage injection was 15 seconds, and the wavelength of the detector was 254 nm . The running time was 20 min at 20℃. Results The average recoveries of adenosine, guanosine, uracil and hypoxanthine was 96 4%,98 2%,94 3% and 95 1%, relative standard deviations were 0 9%,1 2%,1 4% and 1 8%( n =5).Their contents were 0 30%,0 28%,0 14% and 0 05%, respectively. Conclusion The method is simple, rapid and sensitive with good repeatability, and can be used for the quality control of traditional Chinese Cordyceps sinensis .

关键词(KeyWords)： 冬虫夏草;毛细管电泳法;核苷
Cordyceps sinensis (Berk.)Sacc.; capillary zone electrophoresis; nucleosides

Abstract:

Keywords:

基金项目(Foundation):

作者(Authors): 阮婧华,孙毓庆
RUAN Jing hua; SUN Yu qing (School of Pharmacy;

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