药用红景天的品种整理及生药学鉴别研究Classification and pharmacognostic identification of medicinal Rhodiola
宋霞,刘亚蓉,徐智玮,李永鹏,卜晨琛,乔亚玲,王珺,张春平,吴立斌,海平
SONG Xia,LIU Yarong,XU Zhiwei,LI Yongpeng,BU Chenchen,QIAO Yaling,WANG Jun,ZHANG Chunping,WU Libin,HAI Ping
摘要(Abstract):
目的 从标准收载、性状、横切面和粉末显微特征入手对5种常见药用红景天进行比较研究。方法 归纳整理5种红景天的药用标准收载情况;采用性状鉴别法、显微鉴别法(使用生物显微镜、偏光显微镜和荧光显微镜三者结合的方法)对5种红景天进行比较研究。结果 除大花红景天收载于现行《中华人民共和国药典》外,其余四种红景天均收载于地方标准;不同种的红景天性状相似易混淆但存在差异,大花红景天和唐古红景天均具有黄色膜质表皮(唐古红景天黄色膜质表皮为多层);横切面组织构造基本相同,但维管束形式各不相同:大花红景天、狭叶红景天和高山红景天均具有1轮维管束,唐古红景天和长鞭红景天具有2轮维管束,各品种根茎髓部维管束形态各异;粉末特征也有不同:大花红景天、长鞭红景天和高山红景天的薄壁细胞中含大量草酸钙方晶,大花红景天淀粉粒较少且单个散在,其余四种红景天淀粉粒较多且聚集成团。结论 性状、横切面和粉末显微特征为鉴别5种常见药用红景天提供了参考依据。
Objective Five kinds of Rhodiola were compared based on standard collection and microscopic characteristics for transverse section and powder.Methods The medical standard of 5 kinds of Rhodiola was summarized; 5 kinds of medicinal Rhodiola were studied by the method of character identification and microscopic identification(A combination of biological microscope, polarizing microscope and fluorescence microscope).Results The other four species of Rhodiola are all included in local standards except Rhodiola crenulata,which is listed in Chinese pharmacopoeia; The traits of different species of Rhodiola are similar and confusing, but there are differences: Rhodiola crenulata and Rhodiola tangutica have yellow membranous epidermis(yellow membranous epidermis of Rhodiola tangutica is multi-layered);The structure of the cross section is basically the same, but the vascular bundles are different: Rhodiola crenulata,Rhodiola kirilowii and Rhodiola sachalinensis A.Bor.have one round of vascular bundles, Rhodiola tangutica and Rhodiola fastigiata have two rounds of vascular bundles, the vascular bundle in pith of rhizome was different in different varieties; The powder characteristics of each variety are also different: The parenchyma cells of Rhodiola crenulata,Rhodiola fastigiata and Rhodiola sachalinensis A.Bor.contain a large amount of calcium oxalate square crystals, starch grains of Rhodiola crenulata were few and scattered singly, while starch grains of the other four Rhodiola macrophylla species were more and clustered.Conclusion The character identification, transverse section and powder microscopic characteristics provided reference for the identification of 5 common rhodiola.
关键词(KeyWords):
红景天;性状鉴别;显微特征;偏光显微镜;荧光显微镜
Rhodiola;character identification;microscopic characteristic;polarizing microscope;fluorescence microscope
基金项目(Foundation): 青海省地方中藏药材标准物质研制和标定技术规范研究(2020-ZJ-749)
作者(Author):
宋霞,刘亚蓉,徐智玮,李永鹏,卜晨琛,乔亚玲,王珺,张春平,吴立斌,海平
SONG Xia,LIU Yarong,XU Zhiwei,LI Yongpeng,BU Chenchen,QIAO Yaling,WANG Jun,ZHANG Chunping,WU Libin,HAI Ping
DOI: 10.14066/j.cnki.cn21-1349/r.2021.1391
参考文献(References):
- [1] Chinese Pharmacopoeia Commission.Chinese Pharmacopoeia:part 1(中华人民共和国药典:一部)[M].Beijing:China Medical Science Press,2020:161-162.
- [2] Jilin Medical Products Administration.Standard of Chinese Medicinal Materials of Jilin Province:Volume 1(吉林省中药材标准:第一册)[M].Changchun:Jilin Science and Technology Press,2019:217.
- [3] Zhejiang Food and Medical Products Administration.Standard of Chinese Medicinal Materials of Zhejiang Province:Volume 1(浙江省中药材标准:第一册)[M].Hangzhou:Zhejiang People′s Publishing House,2017:33-35.
- [4] XU Z W,JIA S N,LI Y W,et al.Investigation and conservation of medicinal plant resources of Rhodiola L[J].Modern Chinese Medicine(中国现代中药),2019,21(10):1348-1353.
- [5] Qinghai Medical Products Administration.Standard of Chinese Medicinal Materials of Qinghai Province(青海省藏药材标准)[M].Lanzhou:Gansu Nationalities Publishing House,2019:123.
- [6] Sichuan Food and Medical Products Administration.Standard of Chinese medicinal materials of Sichuan Province(四川省藏药材标准)[M].Chendu:Sichuan Science and Technology Press,2014:132.
- [7] Gansu Food and Medical Products Administration.Standard of Chinese Medicinal Materials of Gansu Province(甘肃省中药材标准)[M].Lanzhou:Gansu Culture Publishing House,2009:132.
- [8] Xizang,Qinghai,Sichuan,Gansu,Yunnan,Xinjian Health Bureau.Standard of Tibetan medicine(藏药标准)[M].Xining:Qinghai People′s Publishing House,1979:44.
- [9] Qinghai Food and Medical Products Administration.Tibetan Medicine Processing Standards of Qinghai Province(青海省藏药炮制规范)[M].Xining:Qinghai People′s Publishing House,2010:75.
- [10] Chinese Pharmacopoeia Commission.Chinese Pharmacopoeia:part 1(中华人民共和国药典:一部)[M].Beijing:People′s Sanitary Publishing Press,1978:262.
- [11] Jilin Medical Products Administration.Standard of Medicinal Materials of Jilin Province(吉林省药材标准)[M].Changchun:Jilin Science and Technology Press,2020:217.
- [12] HUANG L Q.Atlas of Medicinal Materials of Chinese Herbal Medicine and Ethnic Medicine(中草药与民族药药材图谱)[M].Beijing:Beijing Medical University Press,2005:23.
- [13] Sichuan Medical Products Administration.Standard of Chinese medicinal Materials of Sichuan Province(四川省藏药材标准)[M].Chendu:Sichuan Science and Technology Press,2020:48.
- [14] Chinese Pharmacopoeia Commission.An Illustrated Handbook on Microscopic Identification of Chinese Crude Drugs for Chinese Pharmacopoeia(中华人民共和国药典中药材显微鉴别彩色图鉴)[M].Beijing:People′s Medical Publishing House,2009:13-15.
- [15] CHEN Y Y,ZHANG Y M,WANG S Y,et al.Location analysis of volatile oil in Angelicae Sinensis Radix by frozen section and fluorescence imaging[J].Chinese Journal of Experimental Traditional Medical Formulae(中国实验方剂学杂志),2022,28(1):182-188.
- [16] CHEN H J.Study on medicinal plant resources of Rhodiola in Qinghai Province(青海红景天属药用植物资源研究)[D].Shenyang:Shenyang Pharmaceutical University,2009.
- [17] WANG Y H,LIU H M,LIU X,et al.Study on preparation and structural observation of paraffin sections of three kinds of Rhodiola [J].Seed(种子),2018,37(7):28-34.
- [18] LIU W Q.Comparative analysis of five Rhodiola Rosea crude drugs[J].Journal of Hunan University of Chinese Medicine(湖南中医药大学学报),2007,27:46-47.
- [19] JIA G F.The plant morphology of Rhodiola crenulata and its medicinal herb appraisal[J].Journal of Grassland and Forage Science(四川草原),2002(4):53-61.
- [20] BAI N,LI T,ZHANG H.Pharmaceutical identification on root of Rhodiola crenulata(Hook.f.et Thoms.)H.Ohba[J].West China Journal of Pharmaceutical Sciences(华西药学杂志),2007,22(4):419-420.
- [21] CHEN Y T,ZHU M P,WANG D H.Review on Rhodiola L.of identification studies[J].Lishizhen Medicine and Materia Medica(时珍国医国药),2007,18(6):1499-1501.
- [22] GU Y L.Study on variety finishing and quality of Rhodiola Rosea(红景天的品种整理和质量研究)[D].Beijing:Beijing University of Chinese Medicine,2003.
- 红景天
- 性状鉴别
- 显微特征
- 偏光显微镜
- 荧光显微镜
Rhodiola - character identification
- microscopic characteristic
- polarizing microscope
- fluorescence microscope
- 宋霞
- 刘亚蓉
- 徐智玮
- 李永鹏
- 卜晨琛
- 乔亚玲
- 王珺
- 张春平
- 吴立斌
- 海平
SONG Xia - LIU Yarong
- XU Zhiwei
- LI Yongpeng
- BU Chenchen
- QIAO Yaling
- WANG Jun
- ZHANG Chunping
- WU Libin
- HAI Ping
- 宋霞
- 刘亚蓉
- 徐智玮
- 李永鹏
- 卜晨琛
- 乔亚玲
- 王珺
- 张春平
- 吴立斌
- 海平
SONG Xia - LIU Yarong
- XU Zhiwei
- LI Yongpeng
- BU Chenchen
- QIAO Yaling
- WANG Jun
- ZHANG Chunping
- WU Libin
- HAI Ping