赵艳秋,马鹤,徐晖,曹双敏,王绍宁,王国清
ZHAO Yan-qiu1,MA He2,XU Hui1,CAO Shuang-min1,WANG Shao-ning2,WANG Guo-qing2(1.School of Pharmacy

• 1:沈阳药科大学药学院
• 2:沈阳药科大学制药工程学院

摘要(Abstract)：

目的考察多孔羟基磷灰石微球对药物的吸附性质。方法采用溶液吸附法研究市售羟基磷灰石微球对药物的吸附性质。以氯化锶为标记物,用扫描电镜-能谱分析仪定点扫描微球内锶的分布情况,氮吸附法测定微球的比表面积和孔径。结果可与羟基磷灰石上的钙离子螯合的药物吸附量相对较高,而依靠氢键或电荷间相互作用、发生物理吸附的药物吸附量偏低,难溶性药物吸附药量普遍低于水溶性药物。市售微球孔径约为30 nm,属于介孔材料,药物多分布在微球的表层。结论阐明了羟基磷灰石微球对不同类型药物的吸附特征,为其作为药物载体提供依据。
Objective To study the drug adsorption properties on porous hydroxyapatite microspheres(HAP-MS).Methods The absorption of various drugs on commercial HAP-MS was examined using solution absorption method.The absorption of strontium on HAP was analyzed using SEM-EDX,and the specific surface area and pore size of HAP-MS were determined with nitrogen adsorption method.Results The amount of drugs adsorbed on HAP-MS was relatively high for drugs which can chelate with calcium ion,while only small amount of drug was adsorbed for drugs which can only physically adsorb via hydrogen bonding or charge interaction.The amount of insoluble drugs adsorbed on HAP-MS was generally lower than that of water soluble drugs.The commercial HAP-MS was mesoporous material with a most probable pore size of 30 nm,and the drugs adsorbed mainly distributed on the outer layer of HAP-MS.Conclusions The drug adsorption properties on HAP-MS are determined,the results may provide valuable implication for the application of HAP-MS in drug delivery.

关键词(KeyWords)： 羟基磷灰石;微球;药物吸附;介孔
hydroxyapatite;microsphere;drug adsorption;mesoporous

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 赵艳秋,马鹤,徐晖,曹双敏,王绍宁,王国清
ZHAO Yan-qiu1,MA He2,XU Hui1,CAO Shuang-min1,WANG Shao-ning2,WANG Guo-qing2(1.School of Pharmacy

DOI: 10.14066/j.cnki.cn21-1349/r.2012.08.007

参考文献(References)：

• [1]ANDERSON J M,SHIVE M S.Biodegradation and bio-compatibility of PLA and PLGA microspheres[J].AdvDrug Deliv Rev,1997,32:231-241.
• [2]KAWASAKI T.Fundamental study of hydroxyapatitehigh performance liquid chromatography[J].Chroma-tography,1990,515:125-128.
• [3]MAA J,WANG C,PENG K W.Electrophoretic deposi-tion of porous hydroxyapatite interface[J].Biomateri-als,2003,24:3505-3510.
• [4]HU Qiao-ling,LI Bao-qiang,WANG Mang,et al.Prepa-ration and characteration of biodegradable chitosan/hydroxyapatite nanocomsite rods via in situ hybridiza-tion:a potential material as fixation of bone fracture[J].Biomaterials,2004,25(5):779-785.
• [5]ZHANG Jing-xian,MAEDA M,KOTOBUKI N,et al.A-queous processing of hydroxyapatite[J].MaterialsChemistry and Physics,2006,99:398-404.
• [6]DAVID M,DUFFY M D.Complication of fillers:Over-view[J].J Dermatol Surg,2005,12:1226-1633.
• [7]郑步中,吕晓迎.羟基磷灰石在口腔保健领域的应用[J].国外医学生物医学工程分册,2004,2(27):26-29.
• [8]HABRAKENW J E M,WOLKE J G C,JANSEN J A.Ceramic composites as matrices and scaffolds for drugdelivery in tissue engineering[J].Advanced Drug De-livery Reviews,2007,59(4-5):234-248.
• [9]GINEBRA M P,TRAYKOVA T.Calcium phosphate ce-ments as bone drug delivery systems:A review[J].JControlled Release,2006,113(2):102-110.
• [10]NIE He-min,WANG Chi-hua.Fabrication and charac-terization of PLGA/HAP composite scaffolds for deliv-ery of BMP-2 plasmid DNA[J].J Controlled Release,2007,120:111-121.
• [11]MASUMOTO T,OKAZAKI M,INOUE M,et al.Hydroxyapatite particles as a controlled release carrier ofprotein[J].Biomaterials,2004,25(17):3807-3812.
• [12]ZHANG Cui-miao,LI Chun-xia,HUANG Shan-shan,etal.Self-activated luminescent and mesoporous strontiumhydroxyapatite nanorods for drug delivery[J].Biomate-rials,2010,31(12):3374-3383.
• [13]赵勤富,王思玲.纳米多孔羟基磷灰石的制备方法及其在药物载体方面应用的研究进展[J].沈阳药科大学学报,2010,27(12):1009-1013.
• [14]WANG Shao-ning,WANG Xiao-yun,XU Hui,et al.Towards sustained delivery of small molecular drugs u-sing hydroxyapatite microspheres as the vehicle[J].Advanced Powder Technology,2010,21:268-272.
• [15]李世普,王友法.磷灰石纳米粒子的制备改性及生物安全性[M].北京:科学出版社,2010:4.