蟾饲五谷虫中华蟾酥毒基的含量测定Quantitative determination of cinobufagin in maggot bred by toad
孙晓亚,刘虹霞,岳亮,沈文娟,张国刚
SUN Xiao-ya,LIU Hong-xia,YUE Liang,SHEN Wen-juan,ZHANG Guo-gang(School of Traditional Chinese Materia Medica
摘要(Abstract)：
目的建立反相高效液相色谱法测定蟾饲五谷虫中华蟾酥毒基的含量。方法采用Ecosil C18色谱柱(250 mm×4.6 mm,5μm),以乙腈-5 g.L-1磷酸二氢钾溶液(体积比38∶62)(用磷酸调节pH值为3.2)为流动相,检测波长为296 nm,柱温为40℃,流速为1.0 mL.min-1。结果华蟾酥毒基的线性范围为6.0～30.0 mg.L-1(r=0.998 7,n=5),平均回收率为100.0%(RSD=1.54%,n=6)。结论该方法操作简便、结果准确,可用于蟾饲五谷虫的质量控制。
Objective To establish an RP-HPLC method for determination of cinobufagin in maggot bred by toad.Methods A column packed with Ecosil C18 column(250 mm×4.6 mm,5 μm)was used as the stationary phase,the mixture of acetonitrile-KH2PO4(the pH of solution was adjusted to 3.2 by phosphoric acid)(V∶ V=38∶ 62)was served as mobile phase.The detection wavelength was set at 298 nm;the column temperature was 30 ℃;the flow rate was 1.0 mL · min-1.Results The linear range of cinobufagin was 6.0-30.0 mg · L-1(r=0.998 7,n=5),the average recovery of cinobufagin was 100.0%(RSD=1.54%,n=6).Conclusions The method is found to be convenient and accurate,and can be used for the quality control of cinobufagin.
关键词(KeyWords)：
蟾饲五谷虫;华蟾酥毒基;含量测定;高效液相色谱
maggot bred by toad;cinobufagin;content determination;HPLC
基金项目(Foundation):
作者(Author):
孙晓亚,刘虹霞,岳亮,沈文娟,张国刚
SUN Xiao-ya,LIU Hong-xia,YUE Liang,SHEN Wen-juan,ZHANG Guo-gang(School of Traditional Chinese Materia Medica
DOI: 10.14066/j.cnki.cn21-1349/r.2012.05.008
参考文献(References)：
- [1]许兵红,陈正跃,艾予川.丝光绿蝇饲养初步研究[J].新乡医学院学报,1999,16(1):30-31.
- [2]谢观.中华医学大辞典[M].沈阳:辽宁科学技术出版社,1994:1366.
- [3]华允芬,吴江林,钱俊青.特种五谷虫脂肪酸的体外抗肿瘤、抗HIV-1整合酶活性及组成分析[J].昆虫学报,2008,2:137-142.
- [4]周学敏,程培元,许美娟.蟾酥成分的分析方法研究[J].中草药,1992,23(9):490.
- [5]伊慧娴,孔令俐.用多次层析法鉴别中药成方制剂中的蟾酥[J].中草药,1997,28(5):277.
- [6]于翠翠,金向群,岳峻威,等.HPLC法测定蒡芩慢咽滴丸中酯蟾毒配基及华蟾酥毒基的含量[J].中国实验方剂学杂志报,2009,15(2):17-19.