韩木南,陈晓辉,纪晓影,其其格,毕开顺
HAN Mu-nan,CHEN Xiao-hui,JI Xiao-ying,QIQI Ge,BI Kai-shun(School of Pharmacy

• 1:沈阳药科大学药学院

摘要(Abstract)：

目的采用柱前衍生高效液相色谱法对L-肌肽原料药中的游离氨基酸进行测定。方法采用异硫氰酸苯酯(phenyl isothiocyanate,PITC)柱前衍生高效液相色谱法,使L-肌肽中游离的β-丙氨酸和L-组氨酸衍生物达到完全分离,并测定其含量。色谱柱为DiamonsilTM C18(200 mm×4.6 mm,5μm),流动相为甲醇-0.05 mol.L-1醋酸钠溶液(体积比为13∶87),流速为1.0 mL.min-1,检测波长为254 nm,柱温为35℃。结果HPLC法测定β-丙氨酸和L-组氨酸的线性范围分别为2.01～20.10 mg.L-1(r=0.999 7)和2.00～20.00 mg.L-1(r=0.999 3);平均回收率分别为110.6%和98.2%。结论HPLC法无需专业的氨基酸分析仪,可用于L-肌肽中游离氨基酸的检查。
Objective To establish an HPLC method with precolumn derivatization for determining the contents of two free amino acids in L-carnosine.Methods Using phenyl isothiocyanate(PITC)as the derivating reagent,the contents of β-alanine and histidine in L-carnosine were determined,which were completely separated.The column was DiamonslTM C18(200 mm×4.6 mm,5 μm);the mobile phase was methanol-0.05 mol·L-1 sodium acetate solution(V∶V=13∶87);the flow rate was 1.0 mL·min-1;the detection wavelength was set at 254 nm and the column temperature was 35 ℃.Results The liner ranges of β-alanine and histidine were 2.01-20.10 mg·L-1(r=0.999 7)and 2.00-20.00 mg·L-1(r=0.999 3),respectively;their average recoveries were 110.6% and 98.2%.Conclusions The method does not need the special amino acid analyzer,and it is efficient,sensitive and accurate for determination of the free amino acids in L-carnosine.

关键词(KeyWords)： L-肌肽;氨基酸;高效液相色谱法
L-carnosine;amino acid;HPLC

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 韩木南,陈晓辉,纪晓影,其其格,毕开顺
HAN Mu-nan,CHEN Xiao-hui,JI Xiao-ying,QIQI Ge,BI Kai-shun(School of Pharmacy

DOI: 10.14066/j.cnki.cn21-1349/r.2010.04.015

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