高效液相色谱梯度洗脱法测定大鼠胆汁中9-硝基喜树碱含量Determination of 9-nitrocamptothecin in the rat′s bile by HPLC using the gradient elution
李柯,徐静华,薛开会,钟大放
LI Ke 1; XU Jing hua 2; XUE Kai hui 1; ZHONG Da fang 1 (1. Laboratory of Drug Metabolism and Pharmacokinetics;
摘要(Abstract)：
目的建立大鼠胆汁中 9 硝基喜树碱的高效液相色谱梯度洗脱方法。方法胆汁样品经乙腈 水 ( 1∶1 )稀释后 ,直接进样。检测波长为 370nm ,采用HypersilBDSC18柱 ( 5 μm,1 5 0mm× 4 6mmI D )分离 ,使用以乙腈 2 %甲酸水溶液为流动相的二元梯度洗脱方法。结果线性范围为 0 5～2 5 μg/mL,定量限为 0 5 μg/mL。结论本法操作简便 ,成功应用于胆汁中 9 硝基喜树碱的含量的测定及胆汁中 9 硝基喜树碱代谢物的初步研究。
Objective To develop a novel HPLC UV method for determination of 9 nitrocamptothecin (9 NC) in the rat′s bile. Method The samples were separated on a Hypersil BDS C 18 column after being diluted by acetonitrile water (1∶1). The mobile phase consisted of acetonitrile and water, and a gradient elution program was applied. The UV detector was set at 370 nm. Result The liner calibration curves were obtained in the concentration range of 0 5 ～ 25 μg/mL . The limit of quantification was 0 5 μg/mL . Conclusion The method was simple and successfully applied to determining the concentrations of 9 NC in the bile samples, and it also offered a way to the preliminary study on the metabolites of 9 NC in the rat′s bile.
关键词(KeyWords)：
9-硝基喜树碱;高效液相色谱法;梯度洗脱;胆汁
nitrocamptothecin; HPLC; gradient elution; bile
基金项目(Foundation): 国家自然科学基金资助项目 ( 39930 1 80 )
作者(Authors):
李柯,徐静华,薛开会,钟大放
LI Ke 1; XU Jing hua 2; XUE Kai hui 1; ZHONG Da fang 1 (1. Laboratory of Drug Metabolism and Pharmacokinetics;
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