梁亚男,姜婉茹,常文惠,左丽娟,杜雯辉,朱勒德斯·阿依恒,孙璐,周地,李宁
LIANG Yanan,JIANG Wanru,CHANG Wenhui,ZUO Lijun,DU Wenhui,ZHU LE DE SI·Ahengyi,SUN Lu,ZHOU Di,LI Ning

• 1:沈阳药科大学中药学院/沈阳市中药药效物质研究与创新药开发重点实验室
• 2:沈阳药科大学辽宁省重大慢病中药创新药重点实验室
• 3:沈阳药科大学药学院
• 4:广西师范大学药用资源化学与药物分子工程国家重点实验室

摘要(Abstract)：

目的 研究玉竹多酚成分及其对二氧化硅(silicon dioxide, SiO_2)诱导的A549细胞损伤的保护作用。方法 结合产率与不同溶剂提取物活性的关系，本研究选定80%甲醇/水为最佳提取溶剂。生物活性导向筛选表明，玉竹总多酚是发挥细胞保护作用的主要活性成分。从玉竹根茎提取物的多酚组分中鉴定化合物，并研究其对SiO_2诱导的A549细胞损伤的保护作用；结果 从玉竹根茎提取物的多酚组分中鉴定出28种化合物，且多数化合物对SiO_2诱导的A549细胞损伤表现出显著的保护活性，其ED_(50)值较低，保护活性优于白藜芦醇(14.7μmol·L~(-1))。进一步进行了活性氧和凋亡测定，结果显示，多酚成分的保护作用可能与其抗氧化活性有关。结论 玉竹(Polygonatum odoratum)是一种传统的食物增补剂，具有扶正固本的作用。作者首次发现玉竹可以保护肺细胞免受环境微粒SiO_2的损伤。玉竹可作为一种潜在的健康膳食来源，并可进一步应用于功能性食品中。
Objective To investigate the polyphenol constituents of Polygonatum odoratum and their cytoprotective activities against SiO_2-induced A549 cells damage.Methods According to the relationship between the yields and the activities of different solvents, 80% MeOH/H_2O was chosen as the best extraction solvent.Bioassay-guided screening proved that total polyphenolics were the major active fraction for the protective effect.Polyphenol compounds were identified from polyphenols fraction of extract of rhizomes of P.odoratum.Protective activities of compounds were assessed in SiO_2-induced A549 cell damage.Results 28 polyphenol compounds were identified from polyphenols fraction of extract of rhizomes of P.odoratum.Many compounds exhibited remarkable protective activity against SiO_2-induced A549 cell damage with low ED_(50) values, outperforming resveratrol(14.7 μmol·L~(-1)).ROS assays and apoptotic assay were further conducted to suggest that protective effects may be related to antioxidant activity.Conclusion Polygonatum odoratum is routinely acted as a traditional food supplement with effects of strengthening and consolidating body resistance.P.odoratum was discovered to protect lung cells from environmental finer particle(SiO_2)-induced injury for the first time.P.odoratum can be served as promising sources of healthy diet and be further applied in functional foods.

关键词(KeyWords)： 玉竹;多酚;保护作用;SiO_2诱导的细胞损伤;A549
Polygonatum odoratum;phenolics;protective effects;SiO_2-induced cell damage;A549

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(81872768);; 辽宁省自然科学基金计划项目(2022-MS-242,2020-BS-129);; 辽宁省教育厅基本科研项目(LJKZ0945);; 沈阳药科大学大学生创新创业训练计划项目(X202110163108)

作者(Author): 梁亚男,姜婉茹,常文惠,左丽娟,杜雯辉,朱勒德斯·阿依恒,孙璐,周地,李宁
LIANG Yanan,JIANG Wanru,CHANG Wenhui,ZUO Lijun,DU Wenhui,ZHU LE DE SI·Ahengyi,SUN Lu,ZHOU Di,LI Ning

DOI: 10.14066/j.cnki.cn21-1349/r.2021.0901

参考文献(References)：

• [1] YAO W X,LI Y,HAN L,et al.The CDR1 as miR-7/TGFBR2 axis modulates EMT in silica-induced pulmonary fibrosis [J].Toxicol Sci,2018,166(2):465-478.
• [2] BAI X,LIU Y,WANG S Q,et al.Ultrafine particle libraries for exploring mechanisms of pm 2.5 -induced toxicity in human cells [J].Ecotoxicol Environ Saf,2018,157:380-387.
• [3] AHAMED M.Silica nanoparticles-induced cytotoxicity,oxidative stress and apoptosis in cultured a431 and A549 cells [J].Hum Exp Toxicol,2013,32(2):186-195.
• [4] LIU X Y,WANG S,LI C J,et al.Dammarane-type saponins from the leaves of Panax notoginseng and their neuroprotective effects on damaged SH-SY5Y cells [J].Phytochemistry,2017,145:10-17.
• [5] FERREIRA T P T,ARANTES A C S D,NASCIMENTO C V M F D,et al.IL-13 immunotoxin accelerates resolution of lung pathological changes triggered by silica particles in mice [J].J Immunol,2013,191(10):5220-5229.
• [6] LU C F,LI L Z,ZHOU W,et al.Silica nanoparticles and lead acetate co-exposure triggered synergistic cytotoxicity in A549 cells through potentiation of mitochondria-dependent apoptosis induction [J].Environ Toxicol Pharmacol,2017,52:114.
• [7] YANG J J,WANG T,LI Y,et al.Earthworm extract attenuates silica-induced pulmonary fibrosis through Nrf2-dependent mechanisms [J].Lab Investig,2016,96(12):1279-1300.
• [8] FAYEULLE N,VALLVERDU-QUERALT A,MEUDEC E,et al.Characterization of new flavan-3-ol derivatives in fermented cocoa beans [J].Food Chem,2018,259:207-212.
• [9] RAI S,KUREEL A K,DUTTA P K,et al.Phenolic compounds based conjugates from dextran aldehyde and BSA:Preparation,characterization and evaluation of their anti-cancer efficacy for therapeutic applications [J].International J Biol Macromol,2018,110:425-436.
• [10] CHE Y Y,QIAN Y,WU Y,et al.Two new homoisoflavanones from the rhizome of Polygonatum odoratum[J].Chem Nat Compd,2015,51(1):54-56.
• [11] WANG D M,LI D W,ZHU W,et al.Steroidal saponins from the rhizomes of Polygonatum odoratum[J].Nat Prod Res,2009,23(10):940-947.
• [12] BAEK S H,JIN G L,PARK S Y,et al.Gas chromatographic determination of azetidine-2-carboxylic acid in rhizomes of Polygonatum sibiricum and Polygonatum odoratum[J].J Food Compos Anal,2012,25(2):137-141.
• [13] LIU Q B,LI W,NAGATA K,et al.Isolation,structural elucidation,and liquid chromatography-mass spectrometry analysis of steroidal glycosides from Polygonatum odoratum[J].J Agric Food Chem,2018,66(2):521-531.
• [14] ZHOU X L,ZHANG Y P,ZHAO H D,et al.Antioxidant homoisoflavonoids from Polygonatum odoratum[J].Food Chem,2015,186:63-69.
• [15] DENG Y,KAI H,YE X,et al.Saponin rich fractions from Polygonatum odoratum (Mill.) Druce with more potential hypoglycemic effects [J].J Ethnopharmacol,2012,141(1):228-238.
• [16] LI C,CHEN J,LU B,et al.Molecular switch role of Akt in Polygonatum odoratum lectin-induced apoptosis and autophagy in human non-small cell lung cancer A549 cells [J].PLoS One,2014,9(7):e101526.
• [17] YU H S,MA B P,KANG L P,et al.Saponins from the processed rhizomes of Polygonatum kingianum[J].Chem Pharm Bull,2009,57(9):1011-1014.
• [18] ZHOU X Q,LIANG H,LU X H,et al.Flavonoids from Scutellaria baicalensis and their bioactivities [J].J Peking Univ,2009,41(5):578-584.
• [19] GUO M L,SHAO C L,LIU X,et al.Study on the chemical constituents of Sargassum pallidum and anti-tumor activities [J].Chinese Traditional and Herbal Drugs(中草药),2009,40(12):1879-1882.
• [20] MA D,PENG S,HAN L F,et al .Isolation and identification of Nelumbinis Folium[J].Journal of Shenyang Pharmaceutical University(沈阳药科大学学报),2014,31(5):355-359.
• [21] ZHANG G,GUO M L,LI Y,et al.Study on chemical constituents of Flos Carthami[J].Former Academic Journal of Second Military Medical University(第二军医大学学报),2005,26(2):220-221.
• [22] SONG L L,LI X E,YAN P F,et al.Study on the chemical constituents of Daphne genkwa[J].Chinese Traditional and Herbal Drugs(中草药),2010,41(4):536-538.
• [23] WEN J B,ZHANG Q W,YIN Z Q,et al.Flavonoids from the seeds of Oroxylum indicum (L.) Vent [J].Chin Pharm J,2011,46(3):170-172.
• [24] WANG M H,LI L Z,SUN J B,et al.A new antioxidant flavone glycoside from Scutellaria baicalensis Georgi [J].Nat Prod Res,2014,28(20):1772-1775.
• [25] JIANG C L,FAN C L,YE W C.Study on the chemical constituent of Cirsium japonicum DC [J].Chinese Traditional and Herbal Drugs(中草药),2006,37(4):510-512.
• [26] LI J,CHEN Q C,LIN T,et al.Chemical constituents in Amaranthus spinosus[J].Chinese Traditional and Herbal Drugs(中草药),2013,44(3):272-276.
• [27] WANG C,YANG Y,MEI Z,et al.Cytotoxic compounds from Laminaria japonica[J].Chem Nat Compd,2013,49(4):699-701.
• [28] WANG J L,CAO G F,DONG L W,et al.Chemical constituents in seed crust of Syringa oblate[J].Chinese Traditional and Herbal Drugs(中草药),2010,41(10):1598-1601.
• [29] XIAO Y H,CAO H,ZHANG G L.Chemical constituents of Debregeasia orientalis[J].Natural Product Research and Development(天然产物研究与开发),2008,20(1):52-55.
• [30] SUN L R,LI X,WANG S X.Two new alkaloids from the rhizome of Polygonatum sibiricum[J].J Asian Nat Prod Res,2005,7(2):127-130.
• [31] CHEN L,SONG Z Y,WANG J J,et al.Studies on the chemical constituents from aerial parts of Gynura divaricate[J].Journal of Chinese Medicinal Materials(中药材),2010,33(3):373-376.
• [32] BIANCO A,MELCHIONI C,RAMUNNO A,et al.Phenolic components of Oleaeuropaea-isolation of tyrosol derivatives [J].Nat Prod Res,2004,18(1):29-32.
• [33] CHEN H Y,ZHOU C X,LOU Y J,et al.Chemical constituents from Elsholtzia blanda[J].China Journal of Chinese Materia Medica(中国中药杂志),2005,30(20):1589-1591.
• [34] MATSUSHIMA T,KOBAYASHI S,WATANABE S.Air-driven potassium iodide-mediated oxidative photocyclization of stilbene derivatives [J].J Org Chem,2016,81(17):7799-7806.
• [35] SHEN X Q,GE R L,WANG J H.Chemical constituents from Choerospondias axilaris (Roxb.) Burtt et Hill [J].Journal of Henan University(Medical Science) [河南大学学报(医学版)],2009,28(3):196-199.
• [36] GOUVEA D P,BERWALDT G A,NEUENFELDT P D,et al.Synthesis of novel 2-aryl-3-(2-morpholinoethyl)-1,3-thiazinan-4-ones via ultrasound irradiation [J].J Braz Chem Soc,2016,27(6):1-10.
• [37] HIROKO T,TAKAHASHI S,HASUMI T.Isolation of 9,10-dihydroxy- 2-decenoic acid from royal jelly and determination of the absolute configuration by chemical synthesis [J].Tetrahedron Asymmetry,2009,20(4):457-460.
• [38] MOMTAZ S,HUSSEI A A,OSTAD S N,et al.Growth inhibition and induction of apoptosis in human cancerous HeLa cells by Maytenus procumbens [J].Food Chem Toxicol,2013,51:38-45.