刘晓敏,刘广洲
LIU Xiaomin,LIU Guangzhou

• 1:吉林医药学院附属医院临床药学科
• 2:吉林医药学院附属医院普通外科

摘要(Abstract)：

目的 采用高效液相色谱，结合多元统计分析，建立加味生化妇康颗粒的HPLC指纹图谱，对羟基苯甲酸，香蒲新苷，刺芒柄花苷和甘草酸的含量测定方法。方法 以对羟基苯甲酸为参照，绘制10批样品的指纹图谱，相似度评价，确定共有峰，运用SPSS 19.0、SIMCA 13.0软件进行聚类分析、主成分分析；最后测定4种主要成分的含量。结果 10批样品共有18个共有峰，相似度均大于0.85;共指认了对羟基苯甲酸、香蒲新苷、刺芒柄花苷和甘草酸4个共有峰。结果显示，10批加味生化妇康颗粒样品可聚为3类，3个主成分的累积方差贡献率为91.0%。对羟基苯甲酸，香蒲新苷，刺芒柄花苷和甘草酸质量浓度分别在4.80～48.00、13.14～134.00、3.20～32.00、12.60～126.00 mg·L~(-1)内与峰面积呈良好的线性关系，相关系数分别为0.999 7、0.999 6、0.999 5、0.999 6,平均回收率分别为92.9%、94.3%、96.9%和95.4%;RSD分别为2.7%、2.1%、2.4%和2.8%(n=9)。结论 建立的分析方法结合多元统计分析和含量测定适用于加味生化妇康颗粒质量质量评价。
Objective The HPLC fingerprint of Jiawei Shenghua Fukang granules was established by high performance liquid chromatography combined with multivariate statistical analysis, and the contents of p-hydroxybenzoic acid, typhaneoside, formononetin and glycyrrhizic acid were determined.Methods Taking p-hydroxybenzoic acid as the reference, the fingerprints of 10 batches of samples were established, and the similarity was evaluated to determine the common peak.The contents of four main components were determined by cluster analysis and principal component analysis with SPSS 19.0 and SIMCA 13.0 software.Results There were 18 common peaks whose similarities were greater than 0.85 in these 10 batches of samples, and four common peaks were identified, including p-hydroxybenzoic acid, typhaneoside, anthocyanin and glycyrrhizic acid.The results showed that 10 batches of Jiawei Shenghua Fukang granules samples could be grouped into three categories, and the cumulative variance contribution rate of the three principal components was 90.938%.The mass concentrations of P-hydroxybenzoic acid, typhaneoside, anthocyanin and glycyrrhizic acid showed a good linear relationship with the peak area within 4.80-48.00,13.14-134.00,3.20-32.00 and 12.60-126.00 mg·L~(-1),respectively.The correlation coefficients were 0.999 7,0.999 6,0.999 6 and the average recoveries were 92.9%,94.3%,96.9% and 95.4% respectively; RSD were 2.7%,2.1%,2.4% and 2.8%(n=9).Conclusion The established analytical method combined with multivariate statistical analysis and content determination is suitable for the quality evaluation of Jiawei Shenghua Fukang granules.

关键词(KeyWords)： 加味生化妇康颗粒;指纹图谱;含量测定;对羟基苯甲酸;刺芒柄花苷;香蒲新苷;甘草酸
Jiawei Shenghua Fukang granules;fingerprint;content determination;P-hydroxybenzoic acid;typhaneoside;formononetin;glycyrrhizic acid

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 刘晓敏,刘广洲
LIU Xiaomin,LIU Guangzhou

DOI: 10.14066/j.cnki.cn21-1349/r.2022.0259

参考文献(References)：

• [1] Chinese Pharmacopoeia Commission.Pharmacopoeia of the People′s Republic of China:Part 1[M].BeiJing:China Pharmaceutical Science and Technology Press(中国医药科技出版社),2020:1029.
• [2] YANG Z Z,JI L,LI E W,et al.HPLC fingerprint analysis and pattern recognition of Spatholobus suberectus[J].Chinese Traditional and Herbal Drugs(中草药),2017,48(21):4530-4536.
• [3] BAI X W,ZHANG W,FEI Z P,et al.Assay of four components in Typhae Pollen by QAMS[J].Journal of Shenyang Pharmaceutical University(沈阳药科大学学报),2018,35(8):628-631,645.
• [4] CHEN L X,LIU B,GUO F X,et al.Optimization of determination method of typhaneoside and isorhamnetin-3-O-neohesperidoside in Pollen Typhae[J].Journal of Beijing University of Traditional Chinese Medicine(北京中医药大学学报),2016,39(4):304-307.
• [5] CHEN J,ZHANG Q,YANG R,et al.Chemical pattern recognition of Glycyrrhiza uralensis Fisch.and its processed products Glycyrrhizae Radix et Rhizoma Praeparata cum melle[J].Chinese Journal of Pharmaceutical Analysis(药物分析杂志),2020,40(11):1963-1976.
• [6] XIE R,HAO D L,YI H,et al.Quality evaluation of standard decoction of typhae pollen[J].Chinese Journal of Information on Traditional Chinese Medicine(中国中医药信息杂志),2021,28(7):88-93.
• [7] NIN X,YANG Z Y,WANG F W,et al.Simultaneous determination of 7 components in Glycyrrhiza uralensis Fisch and its processed products by HPLC[J].Journal of Chinese Medicinal Materials(中药材),2020,43(9):2146-2150.
• [8] LUAN Z X,XU Z H,DONG X K,et al.Determination of glycyrrhizic acid in glycyrrhiza granules by HPLC and its antibacterial activities[J].Chinese Journal of Veterinary Science(中国兽医学报),2018,38(5):974-977.
• [9] SHI H P,BAO B H,HUANG S L,et al.Establishment of HPLC fingerprint,cluster analysis and PLS-DA of ligusticum chuanxiong decoction pieces[J].China Pharmacy(中国药房),2019,30(8):1066-1071.
• [10] YAN Y F,XU S M,LIANG Y C,et al.Content determination of 10 kinds of chemical components in ligusticum chuanxiong under different storage conditions by HPLC[J].China Pharmacy(中国药房),2019,30(6):807-812.
• [11] GAO Y,ZHOU G M,CHEN J H,et al.Determination of six flavonoids in Agrimonia pilosa ledeb by HPLC[J].Food Science(食品科学),2015,36(18):93-96.
• [12] JIAO Y,LIU X Q.Analysis of HPLC fingerprint of Faeces trogopterori[J].Chinese Journal of Experimental Traditional Medical Formulae(中国实验方剂学杂志),2012,18(5):98-100.