曾珂,吴晓骏,曹家庆,何艳妮,赵余庆
ZENG Ke1,WU Xiao-jun2,CAO Jia-qing1,HE Yan-ni1,ZHAO Yu-qing1(1.School of Traditional Chinese Materia Medica

• 1:沈阳药科大学中药学院
• 2:山东药品食品职业学院

摘要(Abstract)：

目的研究苦瓜提取物对高脂膳食诱导肥胖大鼠的减肥作用和可能机制。方法高脂饲料喂养制备肥胖大鼠模型,然后随机分为空白对照组、模型对照组和苦瓜提取物36 g.kg-1治疗组,分别灌胃给予质量分数为0.5%的羧甲基纤维素钠和苦瓜提取物,给药周期为6周,实验过程中每周记录体质量,最后一周测定摄食量,实验结束后检测体内脂肪含量,检测血清高密度脂蛋白(highdensity lipoprotein,HDL)、低密度脂蛋白(low density lipoprotein,LDL)、谷草转氨酶(aspartate ami-no transaminase,AST)和谷丙转氨酶(alanine amino transferase,ALT)水平以及肝脏脂肪病变。结果苦瓜提取物能够在不影响大鼠摄食量的基础上抑制肥胖大鼠的体质量增长速度,降低其体质量和附睾下脂肪量,升高血清中HDL水平,降低血清中AST水平,抑制肝细胞脂肪变性;而对Lee's指数、肠系膜和肾周脂肪量、血清中LDL和ALT水平无明显影响。结论苦瓜提取物具有一定减肥和保肝作用,该作用可能与升高血清HDL水平和降低血清AST水平以及抑制肝细胞脂肪变性有关。
Objective To study the anti-obesity effect of Momordica charantia extract(MCE)on obese rats induced by high-fat diet and possible mechanisms.Methods Rats were randomly divided into the normal control group fed normal diet and model group fed high-fat diet,model group rats were further divided into model control group and MCE group after six weeks when the body weight was higher than normal control group obviously,the rats of MCE group treated with MCE 36 g · kg-1 daily,rats of normal control group and model control group received 0.5% CMC-Na with the same volume.Body weight and food intake of last week after MCE treatment of rats were recorded weekly.The weight of fast rats and fat tissue,the levels of fast serum HDL,LDL,AST,ALT and pathological change of liver were determined six weeks later with HE staining.Results The body weight,body weight gain and epididymal fatty tissue weight were significantly decreased by MCE,the increased serum HDL and decreased serum AST concentration were also observed,the fat accumulation in the liver was reduced obviously in the rats of MCE group compared with model control group.However,there was no significant difference between MCE group and model control group in serum LDL,ALT concentration and Lee′s index.Conclusions The increased serum HDL,decreased serum AST concentration and reduction of fat accumulation in the liver may mediate the anti-obesity and liver protective effects of MCE.

关键词(KeyWords)： 苦瓜;高脂膳食;肥胖大鼠;减肥
Momordica charantia;high-fat diet;obese rat;anti-obesity

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 曾珂,吴晓骏,曹家庆,何艳妮,赵余庆
ZENG Ke1,WU Xiao-jun2,CAO Jia-qing1,HE Yan-ni1,ZHAO Yu-qing1(1.School of Traditional Chinese Materia Medica

DOI: 10.14066/j.cnki.cn21-1349/r.2012.06.005

参考文献(References)：

• [1]SRIDHAR M G,VINAYAGAMOORTHI R,SUY-AMBUNATHAN V A,et al.Bitter gourd(Momordicacharantia)improves insulin sensitivity by increasingskeletal muscle insulin-stimulated IRS-1 tyrosinephosphorylation in high-fat-fed rats[J].Br J Nutr,2008,99(4):806-812.
• [2]CHEN Qi-xuan,LI T S Edmund.Reduced adiposity inbitter melon(Momordica charantia)fed rats is associ-ated with lower tissue triglyceride and higher plasmacatecholamines[J].Br J Nutr,2005,93(5):747-754.
• [3]SHIH Chun-ching,LIN Cheng-shiu,LIN Wei-lin.Effects of Momordica charantia on insulin resistanceand visceral obesity in mice on high-fat diet[J].Dia-betes Res Clin Pract,2008,81(2):134-143.
• [4]AKIHISA T,HIGO N,TOKUDA H,et al.Cucurbi-tane-type triterpenoids from the fruits of Momordicacharantia and their cancer chemopreventive effects[J].J Nat Prod,2007,70(8):1233-1239.
• [5]NERURKAR P V,JOHNS L M,BUESA L M,et al.Momordica charantia(bitter melon)attenuates high-fat diet-associated oxidative stress and neuroinflam-mation[J].J Neuroinflammation,2011,8:64.(doi:10.1186/1742-2094-8-64):1-19.
• [6]陈明之.苦瓜提取物对营养性肥胖小鼠的减肥作用[J].食品研究与开发,2009,30(10):44-46.
• [7]CHEN Qi-xuan,CHAN Laureen L Y,LI T S Ed-mund.Bitter melon(Momordica charantia)reducesadiposity,lowers serum insulin and normalizes glu-cose tolerance in rats fed a high fat diet[J].J Nutr,2003,133(4):1088-1093.
• [8]HUANG Hui-ling,HONG Ya-wen,WONG You-hong,et al.Bitter melon(Momordica charantia L.)inhibits adipocyte hypertrophy and down regulates li-pogenic gene expression in adipose tissue of diet-in-duced obese rats[J].Br J Nutr,2008,99(2):230-239.
• [9]佟丽,陈育尧,陈平雁.清平减肥果茶减肥作用实验研究[J].时珍国医国药,1999,10(9):647-648.
• [10]薛晚利,周玲,李选社,等.丙-聚胶囊对营养性肥胖大鼠的减肥作用[J].西安交通大学学报:医学版,2002,23(6):544-546.
• [11]王慧铭,孙炜,黄素霞,等.昆布多糖对大鼠减肥及降血脂作用的实验研究[J].中国现代应用药学杂志,2008,25(1):16-19.
• [12]宋立人,胡烈,章国镇,等.中华本草:14卷[M].上海:上海科学技术出版社,1999:561.
• [13]DOBRIAN A D,SCHRIVER S D,LYNCH T,et al.Effect of salt on hypertension and oxidative stress in arat model of diet-induced obesity[J].Am J PhysiolRenal Physiol,2003,285(4):F619-F628.
• [14]ASAI A,MIYAZAWA T.Dietary curcuminoids pre-vent high-fat diet induced lipid accumulation in ratliver and epididymal adipose tissue[J].J Nutr,2001,131(11):2932-2935.
• [15]TAKAHASHI Y,IDE T.Dietary n-3 fatty acids affectmRNA level of brown adipose tissue uncoupling pro-tein 1,and white adipose tissue leptin and glucosetransporter 4 in the rat[J].Br J Nutr,2000,84(2):175-184.
• [16]XIE Zuo-quan,LI Hou-kai,WANG Ke,et al.Analy-sis of transcriptome and metabolome profiles altera-tions in fatty liver induced by high-fat diet in rat[J].Metabolism,2010,59(4):554-560.
• [17]RASO G M,ESPOSITO E,IACONO A,et al.Com-parative therapeutic effects of metformin and vitaminE in a model of non-alcoholic steatohepatitis in theyoung rat[J].Eur J Pharmacol,2009,604(1/2/3):125-131.
• [18]VELKOSKA E,WARNER F J,COLE T J,et al.Met-abolic effects of low dose angiotensin converting en-zyme inhibitor in dietary obesity in the rat[J].NutrMetab Cardiovasc Dis,2010,20(1):49-55.
• [19]NERURKAR P V,LEE Y K,NERURKAR V R.Mo-mordica charantia(bitter melon)inhibits primary hu-man adipocyte differentiation by modulating adipo-genic genes[J].BMC Complementary Altern Med,2010,10:34.(doi:10.1186/1472-6882-10-34):1-10.
• [20]冯福建,王五一,虞江萍,等.共轭亚油酸镁硒复方制剂对食高脂饲料肥胖小鼠的减肥作用[J].中国实验方剂学杂志,2008,14(8):35-37.