彭飞,于虹,徐峰,周波,刘德,赵倩
PENG Fei 1; YU Hong 2; XU Feng 1; ZHOU Bo 1; LIU De 1; ZHAO Qian 1 (1.School of Pharmacy;

• 1:沈阳药科大学药学院
• 2:大连美罗药业有限公司
• 3:沈阳药科大学药学院
• 4:沈阳药科大学药学院

摘要(Abstract)：

目的揭示CO2 超临界萃取的中国林蛙卵油 (eggoilofRanatemporariachensinensisDavid ,EORTCD)的镇静和抗惊厥作用。方法通过自主活动监测、协同巴比妥催眠和士的宁诱发惊厥等实验 ,探索中国林蛙卵油的药效和最大效能 ,并与安定、佳静安定进行比较。结果EORTCD对小鼠自主活动有明显的抑制作用 ,对戊巴比妥钠诱导的睡眠有协同效果 ,显著对抗士的宁引起的小鼠惊厥反应 ,通过最大效能比较EORTCD的抗惊厥潜伏期长于安定和佳静安定 ,最大效能三者在同一水平。结论EORTCD具有显著的中枢抑制作用 ,对惊厥动物产生保护作用。
Objective To search the central inhibition effect of the egg oil of Rana temporaria chensinensis David egg oil (EORTCD). Methods The spontaneous activity of mice was monitored, the cooperation of EORTCD with pentobarbital sodium was observed and the convulsant model was induced by strychnine. The pharmacodynamics and efficacy of EORTCD were tested by comparing with Diazepam and Alprazolam. Results EORTCD reduced the locomotor activity of mice, prolonged the sleep duration induced by pentobarbital and exerted an anti convulsion action in strychnine mice. EORTCD showed more effective than Diazepam.and Alprazolam in the prolongation of latency in convulsing mice, and it got the same with Diazepam and Alprazolam in the comparation in efficacy, Conclusions EORTCD indicated a potent anti convulsant effect and protected convulsing animals strongly.

关键词(KeyWords)： 中国林蛙卵油;中枢抑制;镇静;抗惊厥;效能曲线
egg oil of Rana temporaria chensinensis David ; central inhibition; sedation; anti convulsant; efficacy curve

Abstract:

Keywords:

基金项目(Foundation): 辽宁省抚顺市科委资助项目

作者(Authors): 彭飞,于虹,徐峰,周波,刘德,赵倩
PENG Fei 1; YU Hong 2; XU Feng 1; ZHOU Bo 1; LIU De 1; ZHAO Qian 1 (1.School of Pharmacy;

参考文献(References)：

• [1]江苏新医学院.中药大辞典:下册[Z].上海:上海人民出版社,1977.1650.
• [2]李成义,孙文伟,姜熙罗,等.哈士蟆卵油化学成分分析[J].中国药学杂志,1994,29(12):712-713.
• [3]李成义,李静波,李广文,等.吉林产哈士蟆卵油中微量元素含量测定[J].中药材,1991,14(1):16-17.
• [4]徐峰,孙国祥.林蛙卵油的超临界提取工艺[P].中国,发明专利,001233068,2000-11-27.
• [5]徐峰.林蛙卵油软胶囊及其生产工艺[P].中国,发明专利,011062401,2001-2-28.
• [6]卢立明,宋少江,徐绥绪,等.哈士蟆卵油化学成分研究[J].中国药物化学杂志,2001,11(4):224-225.
• [7]CravattBF ,ProsperoGaraciaO ,StuzdakG ,etal.Chemicalcharacterizationofbrainlipidsthatinducesleep[J].Science,1995,268(5216):1506-1509.